斯钙素1在氟中毒大鼠肾损伤中的作用及其与高钙的关系研究

目的观察斯钙素1（STC1）在大鼠氟中毒肾损伤中的作用,并探讨高钙对大鼠肾脏STC1的影响。方法 42只雄性SD大鼠按体重分层分为对照组、低氟组、中氟组、高氟组、低氟加钙组、中氟加钙组、高氟加钙组,每组6只。对照组按体重比注射5ml/kg生理盐水,低、中、高氟组按体重比分别给予5、10、20mg/kg的氟化钠腹腔注射,每48h注射1次,常规自由饲食（含钙量0.87%）;低、中、高氟加钙组除按以上方法染氟外,给予高钙饮食（含钙量5.49%）。建模16周后处死动物,肾脏取材,行免疫组化及RT-PCR检测STC1的定位及表达,以荧光探针标记后采用荧光分光光度仪检测肾细胞内[Ca2＋]i水平,并检测肾...

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Bibliographic Details
Published in解放军医学杂志 Vol. 37; no. 6; pp. 573 - 577
Main Author 张永春 吴小候 罗春丽
Format Journal Article
LanguageChinese
Published 2012
Subjects
斯钙素1
氟中毒
肾脏
高钙血症
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Summary:目的观察斯钙素1（STC1）在大鼠氟中毒肾损伤中的作用,并探讨高钙对大鼠肾脏STC1的影响。方法 42只雄性SD大鼠按体重分层分为对照组、低氟组、中氟组、高氟组、低氟加钙组、中氟加钙组、高氟加钙组,每组6只。对照组按体重比注射5ml/kg生理盐水,低、中、高氟组按体重比分别给予5、10、20mg/kg的氟化钠腹腔注射,每48h注射1次,常规自由饲食（含钙量0.87%）;低、中、高氟加钙组除按以上方法染氟外,给予高钙饮食（含钙量5.49%）。建模16周后处死动物,肾脏取材,行免疫组化及RT-PCR检测STC1的定位及表达,以荧光探针标记后采用荧光分光光度仪检测肾细胞内[Ca2＋]i水平,并检测肾组织脂质过氧化产物的活力。结果 STC1主要定位于肾脏皮髓交界的近曲小管,染氟大鼠的STC1表达量及肾细胞[Ca2＋]i水平显著高于对照组。高钙可增强STC1的表达,使谷胱甘肽过氧化物酶（GSH）、超氧化物歧化酶（SOD）值上升,并抑制[Ca2＋]i水平,使丙二醛（MDA）含量下降,且上述改变随染氟剂量增加更加明显。结论氟中毒肾损伤时STC1可反应性增高,高钙能拮抗氟中毒肾损伤,并促进STC1的表达。STC1可能参与了氟中毒大鼠的肾损伤机制。
Bibliography:11-1056/R
Objective To observe the effect of stanniocalcin-1（STC1） on renal injury of rats with fluorine poisoning,and explore the influence of high calcium diet on STC1 in kidney of rats.Methods Forty two male SD rats were divided into 7 groups（6 each）： control group,low fluoride group,medium fluoride group,high fluoride group,and 3 corresponding fluoride dosage plus calcium groups.Animals in the control group received 5ml/kg normal saline intraperitonealy,and animals in 3 fluoride groups received sodium fluoride（NaF） solution 5,10 and 20mg/kg respectively by intraperitoneal injection,once every 48 hours.The rats were fed with conventional diet（0.87% calcium）.Animals of fluoride plus calcium groups were fed with high-calcium diet（5.49% calcium）.After 16 weeks,the model animals were sacrificed.Expression of STC1 and its distribution in renal tissue were detected by immunohistochemistry and RT-PCR,[Ca2＋]i level of renal cells was determined with fluorescent probe,and vitality of lipid peroxidation products in r
ISSN:0577-7402