A High-Throughput Method for Screening Arabidopsis Mutants with Disordered Abiotic Stress-Induced Calcium Signal

It is established that different stresses cause signal-specific changes in cellular Ca2 ~ level, which function as messengers in modulating diverse physiological processes. These calcium signals are important for stress adaptation. Though numbers of downstream components of calcium signal cascades h...

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Bibliographic Details
Published in遗传学报 Vol. 39; no. 5; pp. 225 - 235
Main Author Zhen Pan Yang Zhao Yuan Zheng Juntao Liu Xiangning Jiang Yan Guo
Format Journal Article
LanguageChinese
Published 2012
Subjects
拟南芥
无序
突变体
筛选
诱导
逆境
钙信号
高通量
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Summary:It is established that different stresses cause signal-specific changes in cellular Ca2 ~ level, which function as messengers in modulating diverse physiological processes. These calcium signals are important for stress adaptation. Though numbers of downstream components of calcium signal cascades have been identified, upstream events in calcium signal remain elusive, specifically components required l＇~~r calcium signal generation due to the lack of high-throughput genetic assay. Here, we report the development of an easy and efficient method in a forward genetic screen for Ca2＋ signals-deficient mutants in Arahidopsis thaliana. Using this method, 121 mutants with disordered NaCI- and H=O2-induced Ca2＋ signals are isolated.
Bibliography:Arabidopsis＂ Mutant screen method; Calcium signal; Abiotic stress
11-5450/R
It is established that different stresses cause signal-specific changes in cellular Ca2 ~ level, which function as messengers in modulating diverse physiological processes. These calcium signals are important for stress adaptation. Though numbers of downstream components of calcium signal cascades have been identified, upstream events in calcium signal remain elusive, specifically components required l＇~~r calcium signal generation due to the lack of high-throughput genetic assay. Here, we report the development of an easy and efficient method in a forward genetic screen for Ca2＋ signals-deficient mutants in Arahidopsis thaliana. Using this method, 121 mutants with disordered NaCI- and H=O2-induced Ca2＋ signals are isolated.
ISSN:1673-8527