Molecular identification and culture trials of Eutreptiella gymnastica （Eutreptiales, Euglenophyceae）

During a survey for marine microalgal resources, we isolated a rare marine euglenoid from the coastal waters of Qingdao, China in 2009, and established a pure culture. Electron microscopic and molecular phylogenetic （18S rDNA and 16S rDNA sequences） analyses revealed a close affinity with Eutreptiel...

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Published in中国海洋湖沼学报：英文版 Vol. 30; no. 3; pp. 446 - 455
Main Author 徐娜 逄少军 单体锋 刘峰 赵小波 高素芹
Format Journal Article
LanguageEnglish
Published 2012
Subjects
rDNA序列
分子鉴定
培养条件
大肠杆菌
文化
最佳生长条件
海洋微藻
试验
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Summary:During a survey for marine microalgal resources, we isolated a rare marine euglenoid from the coastal waters of Qingdao, China in 2009, and established a pure culture. Electron microscopic and molecular phylogenetic （18S rDNA and 16S rDNA sequences） analyses revealed a close affinity with Eutreptiella gymnastica, a bloom-forming species. Different culture conditions were monitored to understand optimal E. gymnastica growth characteristics. The optimal growth conditions in a batch culture of this isolate were 20C, 160 gmol photons/（mE.s） of white light, and a salinity of 10-31. Nutrient experiments demonstrated that growth increased dramatically with a phosphorus concentration greater than 72 gmol/L. Understanding the effect of culture conditions on E. gymnastica may help understanding the blooming mechanism of this alga in its natural environment.
Bibliography:During a survey for marine microalgal resources, we isolated a rare marine euglenoid from the coastal waters of Qingdao, China in 2009, and established a pure culture. Electron microscopic and molecular phylogenetic （18S rDNA and 16S rDNA sequences） analyses revealed a close affinity with Eutreptiella gymnastica, a bloom-forming species. Different culture conditions were monitored to understand optimal E. gymnastica growth characteristics. The optimal growth conditions in a batch culture of this isolate were 20C, 160 gmol photons/（mE.s） of white light, and a salinity of 10-31. Nutrient experiments demonstrated that growth increased dramatically with a phosphorus concentration greater than 72 gmol/L. Understanding the effect of culture conditions on E. gymnastica may help understanding the blooming mechanism of this alga in its natural environment.
37-1150/P
Eutreptiella; microalgal bloom; 18S rDNA; 16S rDNA
ISSN:0254-4059
1993-5005