The Patatin-Containing Phospholipase A pPLAIIα Modulates Oxylipin Formation and Water Loss in Arabidopsis thaliana

The patatin-related phospholipase A （pPLA） hydrolyzes membrane glycerolipids to produce monoacyl compounds and free fatty acids. Phospholipids are cleaved by pPLAIIα at the sn-1 and sn-2 positions, and galactolipids, including those containing oxophytodienoic acids, can also serve as substrates. Abl...

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Published in分子植物 Vol. 5; no. 2; pp. 452 - 460
Main Author Wen-Yu Yang Yong Zheng Sung Chul Bahn Xiang-Qing Pan Mao-Yin Li Hieu Sy Vu Mary R. Roth Brad Scheu Ruth Welt Yue-Yun Hong Xue-Min Wang
Format Journal Article
LanguageChinese
Published 2012
Subjects
n基因
拟南芥
水土流失
游离脂肪酸
磷脂酰胆碱
磷脂酶A
缺失突变体
茉莉酸甲酯
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Summary:The patatin-related phospholipase A （pPLA） hydrolyzes membrane glycerolipids to produce monoacyl compounds and free fatty acids. Phospholipids are cleaved by pPLAIIα at the sn-1 and sn-2 positions, and galactolipids, including those containing oxophytodienoic acids, can also serve as substrates. Ablation of pPLAIIα decreased lysophosphatidylcholine and lysophosphatidylethanolamine levels, but increased free linolenic acid. pPLAIIα-deficient plants displayed a higher level of jasmonic acid and methyl jasmonate, as well as the oxylipin-biosynthetic intermediates 13-hydroperoxylinolenic acid and 12-oxophytodienoic acid than wild-type （WT） plants. The expression of genes involved in oxylipin production was also higher in the pPLAIlec-deficient mutant than in WT plants. The mutant plants lost water more quickly than WT plants. The stomata of WT and mutant plants responded similarly to abscisic acid. In response to desiccation, the mutant and WT leaves produced abscisic acid at the same rate, but, after 4 h of desi
Bibliography:31-2013/Q
patatin-related phospholipase A; oxidative modified lipids; jasmonate synthesis; water loss; Arabidopsis thaliana.
The patatin-related phospholipase A （pPLA） hydrolyzes membrane glycerolipids to produce monoacyl compounds and free fatty acids. Phospholipids are cleaved by pPLAIIα at the sn-1 and sn-2 positions, and galactolipids, including those containing oxophytodienoic acids, can also serve as substrates. Ablation of pPLAIIα decreased lysophosphatidylcholine and lysophosphatidylethanolamine levels, but increased free linolenic acid. pPLAIIα-deficient plants displayed a higher level of jasmonic acid and methyl jasmonate, as well as the oxylipin-biosynthetic intermediates 13-hydroperoxylinolenic acid and 12-oxophytodienoic acid than wild-type （WT） plants. The expression of genes involved in oxylipin production was also higher in the pPLAIlec-deficient mutant than in WT plants. The mutant plants lost water more quickly than WT plants. The stomata of WT and mutant plants responded similarly to abscisic
ISSN:1674-2052
1752-9867