丁胱亚磺酰亚胺联合放射线对食管癌细胞株TE-1的增敏效应

目的研究丁胱亚磺酰亚胺（buthionine sulfoximine,BSO）联合放射线对食管癌细胞株TE-1放射增敏的影响。方法四甲基偶氮唑蓝比色实验（MTT法）观察BSO及放射线对细胞增殖的抑制作用及BSO对食管癌细胞株TE-1的放射增敏作用。流式细胞法观察BSO、放射线对细胞凋亡及细胞周期的影响。反转录聚合酶链反应（reverse transcription polymerase chine reaction,RT-PCR）、Western blot法观察BSO对锰超氧化物歧化酶（manganese superoxide dismutase,MnSOD）mRNA及蛋白表达的影响。结果 B...

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Bibliographic Details
Published in肿瘤防治研究 Vol. 39; no. 3; pp. 245 - 249
Main Author 孙国贵 胡万宁 于秀荣 李成林 杨从容 王雅棣
Format Journal Article
LanguageChinese
Published 2012
Subjects
丁胱亚磺酰亚胺
放射增敏
锰超氧化物歧化酶
食管肿瘤
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Summary:目的研究丁胱亚磺酰亚胺（buthionine sulfoximine,BSO）联合放射线对食管癌细胞株TE-1放射增敏的影响。方法四甲基偶氮唑蓝比色实验（MTT法）观察BSO及放射线对细胞增殖的抑制作用及BSO对食管癌细胞株TE-1的放射增敏作用。流式细胞法观察BSO、放射线对细胞凋亡及细胞周期的影响。反转录聚合酶链反应（reverse transcription polymerase chine reaction,RT-PCR）、Western blot法观察BSO对锰超氧化物歧化酶（manganese superoxide dismutase,MnSOD）mRNA及蛋白表达的影响。结果 BSO能抑制食管癌TE-1细胞的增殖,具有明显的量效和时效关系;BSO对食管癌TE-1细胞有明显的放射增敏作用;BSO与放射线联合作用于TE-1细胞后,G2/M期细胞比例及凋亡率明显增加,MnSOD mRNA及蛋白表达下降。结论 BSO可能通过影响TE-1细胞周期,降低MnSOD mR-NA及蛋白的表达,从而抑制食管癌细胞增殖、诱导凋亡来增强放射线对食管癌细胞的杀伤作用。
Bibliography:42-1241/R
Buthionine sulfoximine; Manganese superoxide dismutase; Esophageal carcinoma; Radiosensitivity
Sun Guogui1,Hu Wanning1,Yu Xiurong2,Li Chenglin3,Yang Congrong4,Wang Yadi 3 1.Department of Radiochemotherapy,Tangshan People＇s Hospital,Tangshan 063000,China;2.Department of Radiotherapy,The Central Hospital of Luoyang;3.Department of Radiotherapy,The Military General Hospital of Beijing PLA;4.Department of Radiotherapy,The Fourth Hospital of Hebei Medical University
Objective To explore radiosensitizative effect on esophageal cell line TE-1 by buthionine sulfoximine（BSO）combined with radiation.Methods Colorimetric 3-[4,5-dimethy thiazol-2-yl]-2,5-diphenyltetrazolium bromide（MTT）assay was used to study the inhibitory effect and rediosensitivity on proliferation of TE-1 cell treated with BSO and radiation.Additionally,flow cytometry,reverse transcription polymerase chine reaction（RT-PCR）and Western blot were also conducted to observe the influence of BSO and radiation on cell apoptosis,cell cycle and the expr
ISSN:1000-8578