A novel sulfonamide agent, MPSP-O01, exhibits potent activity against human cancer cells in vitro through disruption of microtubule

• Published in: 中国药理学报：英文版 Vol. 33; no. 2; pp. 261 - 270
• Main Author: Zu-long LIU Wei TIAN Yong WANG Shan KUANG Xiao-min LUO Qiang YU
• Format: Journal Article
• Language: English
• Published: 2012
• Subjects: A549细胞; HeLa细胞; HepG2细胞; MCF-7细胞; 人类; 体外抗肿瘤; 微管蛋白; 磺胺剂
• ISSN: 1671-4083; 1745-7254

Aim： To evaluate the anti-cancer effects of a new sulfonamide derivative, 2-（N-（3-chlorophenyl）-4-methoxyphenylsulfonamido）-N-hydrox- ypropanamide （MPSP-O01）. Methods： Human cancer cell lines （HepG2, THP-1, K562, HGC-27, SKOV3, PANC-1, SW480, Kba, HeLa, A549, MDA-MB-453, and MCF- 7） were examined. The cytotoxicity of MPSP-O01 was evaluated using the WST-8 assay. Cell cycle distribution was examined with flow cytometry. Mitotic spindle formation was detected using immunofluorescence microscopy. Apoptosis-related proteins were examined with Western blot using specific phosphorylated protein antibodies. Competitive tubulin-binding assay was performed to test whether the compound competitively bound to the colchicine site. Molecular docking was performed to explore the possible binding conforma- tion. Results： MPSP-O01 potently inhibited the growth of the 12 different types of human cancer cells with the ICso values ranging from 1.9 to 15.7 pmol/L. The compound exerted potent inhibition on the drug-resistant Kb/VCR and MCF-7/ADR cells, as on Kba and MCF-7 cells. In HeLa, HGC-27, A549, and other cells, the compound （5 pmol/L） caused cell cycle arrest at the G2/M phase, and subsequently induced cell apoptosis. In Hela cells, it prevented the mitotic spindle formation. Furthermore, the compound dose-dependently inhibited polymerization of tubulin in vitro, and directly bound to the colchicine-site of 13-tubulin. Molecular docking predicted that the compound may form two hydrogen bonds to the binding pocket. The compound showed synergistic effects with colchicine and taxol in blocking mitosis of HeLa cells. Conclusion： MPSP-O01 shows a broad-spectrum of anti-tumor efficacy in vitro and represents a novel structure with anti-microtubule activity.