高原肺水肿的血浆蛋白质组学研究

目的探讨高原肺水肿患者血浆蛋白组中差异表达的蛋白质及其意义。方法抽取拉萨市（海拔3658m）6例高原肺水肿患者和6例健康对照者的血浆,去除血浆高丰度蛋白,采用2-DE分离血浆/血清低丰度蛋白质,酶解差异表达的蛋白质斑点,采用MALDI-TOF/MS检测肽指纹谱,并在GenBank数据库中进行比对分析。结果与对照组比较,高原肺水肿患者免疫球蛋白κ1轻链、血清转铁蛋白前体、α-胰蛋白酶抑制剂重链相关蛋白表达上调,人纤维胶凝蛋白3表达下调。结论高原肺水肿患者血浆中上述4种蛋白的差异表达可能与高原肺水肿的发生有关,有可能成为预测该疾病的新靶点。...

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Bibliographic Details
Published in解放军医学杂志 Vol. 37; no. 1; pp. 31 - 33
Main Author 罗勇军 陈郁 高钰琪
Format Journal Article
LanguageChinese
Published 2012
Subjects
生物标记
蛋白质组学
血浆
高原肺水肿
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Summary:目的探讨高原肺水肿患者血浆蛋白组中差异表达的蛋白质及其意义。方法抽取拉萨市（海拔3658m）6例高原肺水肿患者和6例健康对照者的血浆,去除血浆高丰度蛋白,采用2-DE分离血浆/血清低丰度蛋白质,酶解差异表达的蛋白质斑点,采用MALDI-TOF/MS检测肽指纹谱,并在GenBank数据库中进行比对分析。结果与对照组比较,高原肺水肿患者免疫球蛋白κ1轻链、血清转铁蛋白前体、α-胰蛋白酶抑制剂重链相关蛋白表达上调,人纤维胶凝蛋白3表达下调。结论高原肺水肿患者血浆中上述4种蛋白的差异表达可能与高原肺水肿的发生有关,有可能成为预测该疾病的新靶点。
Bibliography:high altitude pulmonary edema; proteomics; biological markers; plasma
11-1056/R
LUO Yong-jun, CHEN Yu, GAO Yu-qi 1Department of High Altitude Disease, 2Department of Pathophysiology and High Altitude Pathophysiology, College of High Altitude Military Medicinej Third Military Medical Universityy Chongcling 400038, China
Objective To investigate the differential expressions of protein in the plasma proteome in patients suffering from high altitude pulmonary edema （HAPE） and their implications. Methods The plasma of six HAPE patients and six healthy controls was studied. The high-abundant proteins in plasma were removed. The low-abundant proteins in the plasma/serum were segregated by 2-DE. MALDI-TOF/MS was adopted to measure the peptide dactylogram after the differential proteins spots were digested by enzymes. Comparison and analysis were made in the GeneBank. Results The immunoglobulin K1 light chain, serum transferrin protein precursorj and oe -trypsin inhibitor heavy chain-related protein expression were incre
ISSN:0577-7402