应用巢式PCR-DGGE技术分析稻虱缨小蜂体内Wolbachia的多样性

以采集自中国杭州和菲律宾的稻虱缨小蜂Anagrus nilaparvatae为研究对象,采用巢式PCR扩增Wolbachia的16S rDNA和wsp基因片段,并用DGGE分析稻虱缨小蜂体内Wolbachia的多样性。基于16S rDNA基因的分析结果准确地检测到稻虱缨小蜂体内细菌主要是Acinetobacter sp.,Methylophilus sp.,Acidovorax sp.,Burkholderia sp.和Wolbachia sp.。基于wsp基因的分析结果显示,杭州种群感染的Wolbachia属于A组的Mors亚组,菲律宾种群感染的Wolbachia属于A组的Dro亚组。结果说...

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Bibliographic Details
Published in昆虫学报 Vol. 54; no. 12; pp. 1354 - 1360
Main Author 刘淑平 王新 徐红星 汤江武 郑许松 杨亚军 吕仲贤
Format Journal Article
LanguageChinese
Published 2011
Subjects
16S
PCR-DGGE
rDNA基因
Wolbachia
wsp基因
多样性
稻虱缨小蜂
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ISSN0454-6296

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Summary:以采集自中国杭州和菲律宾的稻虱缨小蜂Anagrus nilaparvatae为研究对象,采用巢式PCR扩增Wolbachia的16S rDNA和wsp基因片段,并用DGGE分析稻虱缨小蜂体内Wolbachia的多样性。基于16S rDNA基因的分析结果准确地检测到稻虱缨小蜂体内细菌主要是Acinetobacter sp.,Methylophilus sp.,Acidovorax sp.,Burkholderia sp.和Wolbachia sp.。基于wsp基因的分析结果显示,杭州种群感染的Wolbachia属于A组的Mors亚组,菲律宾种群感染的Wolbachia属于A组的Dro亚组。结果说明,巢式PCR-DGGE是寄生蜂体内Wolbachia检测和多样性分析的有效方法,其中16S rDNA基因是检测Wolbachia存在的较佳分子标记,wsp基因是Wolbachia多样性分析以及种属鉴定和分型的较佳分子标记。
Bibliography:11-1832/Q
LIU Shu-Ping, WANG Xin, XU Hong-Xing , TANG Jiang-Wu , ZHENG Xu-SongI , YANG Ya- JunI, Li Zhong-Xiant （1. Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China ; 2. School of Life and Environment Science, Hangzhou Normal University, Hangzhou 310036, China）
Anagrus nilaparvatae; Wolbachia; diversity; 16S rDNA gene; wsp gene; PCR-DGGE
The objective of this research is to analyze the diversity of Wolbachia in insects by using nested PCR-DGGE.Samples of Anagrus nilaparvatae,one of dominant egg parasitoids of rice planthoppers in Asian rice growth area,were collected from Hangzhou,China and the Philippines.After the total DNA was extracted,the 16S rDNA and wsp gene fragments of Wolbachia were amplified with nested PCR,and then analyzed using DGGE.The results showed that Wolbachia in A.nilaparvatae were sensitively and exactly detected based on 16S rDNA gene,and the dominant bacteria in A.nilaparvatae were Acinetobacter sp.,Methylophilus sp.,Acidovo
ISSN:0454-6296