Effects of electroacupuncture at Zusanli （ST 36） on neurons in the colonic myenteric plexus in rats with irritable bowel syndrome with constipation

We studied the effects of electroacupuncture at Zusanli （ST 36） on neurons in the colonic myenteric plexus and on defecation in rats with irritable bowel syndrome with constipation （IBS-C）. We also used intragastric administration of pinaverium bromide as a positive control treatment to reveal the p...

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Published in中国神经再生研究：英文版 Vol. 6; no. 33; pp. 2605 - 2609
Main Author Liting Zhu Zhonghan Li Bin Xu Chen Xia Jie Cheng Xiaoren Xiang Yi Zhu
Format Journal Article
LanguageEnglish
Published 2011
Subjects
便秘
免疫组化染色
大鼠
神经元
结肠
综合征
肌间神经丛
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Summary:We studied the effects of electroacupuncture at Zusanli （ST 36） on neurons in the colonic myenteric plexus and on defecation in rats with irritable bowel syndrome with constipation （IBS-C）. We also used intragastric administration of pinaverium bromide as a positive control treatment to reveal the pathway mediating the onset of IBS-C. Both electroacupuncture and pinaverium bromide greatly improved defecation in rats with IBS-C. Immunohistochemical staining of the enteric nervous system neuronal marker protein gene product 9.5 in the colonic myenteric plexus showed that electroacupuncture by itself, or in combination with pinaverium bromide, increased the number of neurons and the staining intensity of protein gene product 9.5 in the colonic myenteric plexus. We conclude that visceral hypersensitivity is likely to be a primary cause of constipation in IBS-C rats.
Bibliography:Liting Zhu,Zhonghan Li,Bin Xu,Chen Xia,Jie Cheng,Xiaoren Xiang,Yi Zhu（ 1.Department of Acupuncture and Massage,Second Clinical College,Nanjing University of Traditional Chinese Medicine,Nanjing 210046,Jiangsu Province,China; 2.National Key Laboratory of Acupuncture and Medicine of China ,Nanjing 210029,Jiangsu Province,China; 3.Department of Acupuncture and Rehabilitation,Jiangsu Province Chinese Medicine Hospital,Nanjing 210029,Jiangsu Province,China;）
electroacupuncture; irritable bowel syndrome with constipation; colonic myenteric plexus; neurons; pinaverium bromide; defecation; neural regeneration
We studied the effects of electroacupuncture at Zusanli （ST 36） on neurons in the colonic myenteric plexus and on defecation in rats with irritable bowel syndrome with constipation （IBS-C）. We also used intragastric administration of pinaverium bromide as a positive control treatment to reveal the pathway mediating the onset of IBS-C. Both electroacupuncture and pinaverium bromide greatly improved defecation in rats with IBS-C. Immunohistochemical staining of the enteric nervous system neuronal marker protein gene product 9.5 in the colonic myenteric plexus showed that electroacupuncture by itself, or in combination with pinaverium bromide, increased the number of neurons and the staining intensity of protein gene product 9.5 in the colonic myenteric plexus. We conclude that visceral hypersensitivity is likely to be a primary cause of constipation in IBS-C rats.
11-5422/R
ISSN:1673-5374