Single Nucleotide Polymorphisms （SNPs） and Variable Number Tandem Repeats （VNTRs） in mtDNA D-loop and CO Ⅱ- tRNA^Lys Intergenic Region with PCOS

Objective To explore the relationships intergenic region in mtDNA with PCOS. of variations in D-loop and COH-tRNA^Lys Methods A total of 77 PCOS and 45 non-PCOS patients were enrolled, whose D-loop and COH-tRNA^Lys intergenic region in mtDNA were amplified and sequenced; sexual hormone assay, oral g...

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Published in生殖与避孕：英文版 Vol. 22; no. 3; pp. 129 - 138
Main Author Zhi-ping HU Ying WANG Wen-wei MAO Xiao-wei ZHANG Jie QIAO Qiu-fang ZHANG
Format Journal Article
LanguageEnglish
Published 2011
Subjects
SNP
tRNA
串联重复
单核苷酸多态性
基因突变
线粒体DNA
赖氨酸
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Summary:Objective To explore the relationships intergenic region in mtDNA with PCOS. of variations in D-loop and COH-tRNA^Lys Methods A total of 77 PCOS and 45 non-PCOS patients were enrolled, whose D-loop and COH-tRNA^Lys intergenic region in mtDNA were amplified and sequenced; sexual hormone assay, oral glucose tolerance test （OGTT） and insulin releasing test were carried out. Then variations found in mtDNA were compared between the two groups, the correlations between variations and clinical indexes were analyzed in all subjects. Results Nucleotide variations found in mtDNA were not different between the two groups, but the mutation of 16 094T/C was found associated with the serum levels ofT and fasting insulin; （303-317）CnTC, associated with the serum levels of A and LH; 195C/T with A level and 491T/C with LH level; （8 272-8 289）（ACCCCCTCT）, was associated with the serum level of 1 h glucose. Conclusion Noncoding region mutations in mtDNA perhaps associate with PCOS clinical symptoms and involve in PCOS development.
Bibliography:Objective To explore the relationships intergenic region in mtDNA with PCOS. of variations in D-loop and COH-tRNA^Lys Methods A total of 77 PCOS and 45 non-PCOS patients were enrolled, whose D-loop and COH-tRNA^Lys intergenic region in mtDNA were amplified and sequenced; sexual hormone assay, oral glucose tolerance test （OGTT） and insulin releasing test were carried out. Then variations found in mtDNA were compared between the two groups, the correlations between variations and clinical indexes were analyzed in all subjects. Results Nucleotide variations found in mtDNA were not different between the two groups, but the mutation of 16 094T/C was found associated with the serum levels ofT and fasting insulin; （303-317）CnTC, associated with the serum levels of A and LH; 195C/T with A level and 491T/C with LH level; （8 272-8 289）（ACCCCCTCT）, was associated with the serum level of 1 h glucose. Conclusion Noncoding region mutations in mtDNA perhaps associate with PCOS clinical symptoms and involve in PCOS development.
31-1555/R
PCOS; D-loop region; COII-tRNATM intergenic region; mtDNA
ISSN:1001-7844