COPPER AMINE OXIDASE1 （CuAO1） of Arabidopsis thaliana Contributes to Abscisic Acid- and Polyamine-lnduced Nitric Oxide Biosynthesis and Abscisic Acid Signal Transduction

• Published in: 分子植物 Vol. 4; no. 4; pp. 663 - 678
• Main Author: Rinukshi Wimalasekera Corina Villar Tahmina Begum Giinther F. E. Scherer
• Format: Journal Article
• Language: Chinese
• Published: 2011
• Subjects: ABA诱导; 一氧化氮; 信号转导网络; 多胺氧化酶; 拟南芥; 生物合成; 脱落酸; 铜
• ISSN: 1674-2052; 1752-9867

Polyamines （PA）, polyamine oxidases, copper amine oxidases, and nitric oxide （NO） play important roles in physiology and stress responses in plants. NO biosynthesis as a result of catabolism of PA by polyamine oxidases and copper amine oxidases may explain in part PA-mediated responses. Involvement of a copper amine oxidase gene, COPPER AMINE OXIDASEI （CuAO1）, of Arabidopsis was tested for its role in stress responses using the knockouts cuao1-1 and cuaol-2. PA-induced and ABA-induced NO production investigated bY fluorometry and fluorescence microscopy showed that the cuaol-1 and cuaol-2 are impaired in NO production, suggesting a function of CuAO1 in PA and ABA-mediated NO production. Furthermore, we found a PA-dependent increase in protein S-nitrosylation. The addition of PA and ABA also resulted in H2O2 increases, cuao1-1 and cuao1-2 showed less sensitivity to exogenous ABA supplementation during germination, seedling establishment, and root growth inhibition as compared to wild-type. In response to ABA t