miR-199a-3p对前列腺癌细胞迁移及侵袭能力的影响

目的从miR-199a-3p在前列腺癌高、低转移潜能细胞中的表达差异出发,研究其在高转移前列腺癌细胞株1E8细胞运动转移中的作用及可能的分子机制。方法运用Realtime PCR法检测miR-199a-3p在前列腺癌高、低转移潜能配对细胞系中的表达差异。通过划痕实验及transwell实验观察1E8细胞及转染miR-199a-3p mimics及mimics NC后该细胞运动迁移能力的变化。结果 （1）RealtimePCR结果显示高转移潜能1E8细胞中miR-199a-3p表达水平明显低于低转移潜能2B4细胞。（2）上调miR-199a-3p会减弱1E8细胞的运动转移能力。结论 miR-19...

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Bibliographic Details
Published in肿瘤防治研究 Vol. 38; no. 8; pp. 875 - 877
Main Author 孔繁飞 王中显 孙朝阳 吕煊 翁丹卉 卢运萍 陈刚 吴明富
Format Journal Article
LanguageChinese
Published 2011
Subjects
microRNA
miR-199a-3p
前列腺癌
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Summary:目的从miR-199a-3p在前列腺癌高、低转移潜能细胞中的表达差异出发,研究其在高转移前列腺癌细胞株1E8细胞运动转移中的作用及可能的分子机制。方法运用Realtime PCR法检测miR-199a-3p在前列腺癌高、低转移潜能配对细胞系中的表达差异。通过划痕实验及transwell实验观察1E8细胞及转染miR-199a-3p mimics及mimics NC后该细胞运动迁移能力的变化。结果 （1）RealtimePCR结果显示高转移潜能1E8细胞中miR-199a-3p表达水平明显低于低转移潜能2B4细胞。（2）上调miR-199a-3p会减弱1E8细胞的运动转移能力。结论 miR-199a-3p的上调可以抑制前列腺癌细胞的运动迁移能力。
Bibliography:42-1241/R
Prostate cancer; miR-199a-3p; microRNA
KONG Fan-fei,WANG Zhong-xian,SUN Chao-yang,LV Xuan,WENG Dan-hui,LU Yun-ping,CHEN Gang,WU Ming-fu Department of Gymecology and Obstetrics Cancer Biology Research Center,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China
Objective To investigate the different expression of miR-199a-3p in different prostate cancer cell lines and the effects of miR-199a-3p on cell migration and invasion in prostate cancer.Methods The expression of miR-199a-3p in 1E8（high metastasis prostate cancer） and 2B4（low metastasis prostate cancer） cell lines were identified by realtime PCR.The migration and invasion abilities were detected by Transwell experiment.Results（1）By comparing with 2B4 cells,of miR-199a-3p was decreased in 1E8 cells.（2）The invasiveness of 1E8 cells were decreased through miR-199a-3p upregulation.Conclusion miR-199a-3p invasiveness of prostate cancer cells.
ISSN:1000-8578