菲和苯并（b）荧蒽曝露对翡翠贻贝外套膜的氧化胁迫及损伤
实验室条件下研究了不同质量浓度（2.0μg.L-1、10.0μg.L-1和50.0μg.L-1）的菲（PHE）和苯并（b）荧蒽（BbF）胁迫15 d和清洁海水恢复7 d中翡翠贻贝（Perna viridis）外套膜组织中超氧化物歧化酶（SOD）活力和丙二醛（MDA）质量摩尔浓度的变化。结果表明,PHE胁迫下翡翠贻贝外套膜SOD活力呈先升高后降低的变化规律;BbF胁迫下的翡翠贻贝外套膜SOD活力在胁迫第15天时被显著诱导（P〈0.05）。从b（MDA）的变化来看,PHE和BbF均可导致翡翠贻贝外套膜明显的氧化损伤,之后在清洁海水的净化过程中,这种损伤逐渐降低并恢复至正常水平。鉴于2种PAHs胁迫...
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| Published in | 南方水产科学 Vol. 7; no. 4; pp. 24 - 29 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese |
| Published |
2011
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| Subjects | |
| Online Access | Get full text |
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| Summary: | 实验室条件下研究了不同质量浓度（2.0μg.L-1、10.0μg.L-1和50.0μg.L-1）的菲（PHE）和苯并（b）荧蒽（BbF）胁迫15 d和清洁海水恢复7 d中翡翠贻贝（Perna viridis）外套膜组织中超氧化物歧化酶（SOD）活力和丙二醛（MDA）质量摩尔浓度的变化。结果表明,PHE胁迫下翡翠贻贝外套膜SOD活力呈先升高后降低的变化规律;BbF胁迫下的翡翠贻贝外套膜SOD活力在胁迫第15天时被显著诱导（P〈0.05）。从b（MDA）的变化来看,PHE和BbF均可导致翡翠贻贝外套膜明显的氧化损伤,之后在清洁海水的净化过程中,这种损伤逐渐降低并恢复至正常水平。鉴于2种PAHs胁迫下翡翠贻贝外套膜SOD活力和b（MDA）均发生明显变化并表现出一定的差异性,翡翠贻贝体内的生化指标适合指示PAHs对海洋环境的污染。 |
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| Bibliography: | The SOD activity and MDA content in mantle were studied after Perna viridis exposing to different concentrations（2.0 μg·L-1,10.0 μg·L-1 and 50.0 μg·L-1） of phenanthrene（PHE）and benzo（b） fluoranthene（BbF） separately under experimental conditions.The results indicate that the SOD activity follows an induced-inhibited trend with phenanthrene exposure,and it is significantly induced by exposure to benzo（b） fluoranthene at 15th day（P0.05）.The change in MDA content suggests both PHE and BbF exposure may cause oxidative damage to the mantle,but in clean water,the damage is ameliorated gradually until it returns to normal.Since SOD activity and MDA content in the mantle of P.viridis change obviously and differently with 2 kinds of PAHs exposure,the biochemical indicators for P.viridis are suitable to indicate the pollution to the marine environment by PAHs. Perna viridis; mantle; phenanthrene（PHE）; benzo（b） fluoranthene（BbF）; superoxide dismutase（SOD）; malondialdehyde（MDA） YANG Tao,CHEN Haigang,CAI Wengui,QIN Jiefang,J |
| ISSN: | 2095-0780 |