Effect of San＇ao Decoction（三拗汤） on the Airway Inflammation and Hyperresponsiveness in a Murine Model of Lipopolysaccharide-enhanced Asthma

• Published in: 中国结合医学杂志：英文版 Vol. 17; no. 7; pp. 537 - 541
• Main Author: 顾鹏程 范欣生 蒋辰雪 许惠琴 俞晶华 唐于平
• Format: Journal Article
• Language: English
• Published: 2011
• Subjects: 哮喘; 小鼠模型; 支气管肺泡灌洗液; 气道反应性; 炎症; 白细胞介素4; 脂多糖; 高反应性
• ISSN: 1672-0415; 1993-0402

Objective：San＇ao Decoction（三拗汤,SAD）,as a representative Chinese medicine（CM）formula, was chosen to evaluate the effect of airway inflammation and hyperresponsiveness on the lipopolysaccharide （LPS）enhanced asthma model.Methods：The asthma model was reproduced in the Balb/C mice sensitized by ovalbumin（OVA）,challenged by OVA and LPS.After Balb/C mice＇s administration of a dose（0.0024 g/kg）of dexamethasone acetate,and three doses（2.2 g/kg,4.4 g/kg and 8.8 g/kg）of SAD,airway inflammation and responsiveness were observed.The airway inflammation was detected by counting bronchoalveolar lavage fluid （BALF）cells and lung histopathology.Also,differential expressions of interferon-r（IFN-γ）,interleukin-4（IL-4）, and IL-5 in the supernatants of BALF were examined.The changes in airway responsiveness indicated by lung resistance（R_L）and stimulated by acetylcholine（Ach）were determined.Results：Small-dose SAD hardly inhibit airway inflammation or hyperresponsiveness in the LPS-enhanced asthma,while medium-dose and high-dose SAD significantly inhibited the airway hyperresponsiveness,and to some extent,reduced airway inflammation. Meanwhile,the small-dose,medium-dose,and high-dose SAD promoted Th1-type cytokines（IFN-γ）and reduced Th2-type cytokines（IL-4,IL-5）to different extents,which led to a Th1/Th2 balance.Conclusion：SAD has a good therapeutic effect on airway hyperresponsiveness in the LPS-enhanced asthma model,but its definite influence on airway inflammation is not remarkable.