JAK2/STAT3信号通路在重症急性胰腺炎大鼠肺损伤中的作用

目的探讨JAK2/STAT 3信号通路在实验性重症急性胰腺炎（SAP）肺损伤中的作用。方法以4%牛磺胆酸钠胰胆管逆行注射诱导大鼠SAP模型。32只雄性SD大鼠随机分为4组：对照组（NC组）和SAP 6h、12h、18h组,每组8只。动态测定各组血清淀粉酶（AMY）水平;光镜下观察胰腺及肺组织病理变化,并计算肺湿/干重比;ELISA法检测血清IL-6及IL-18表达情况;West-ern blotting检测肺组织中JAK2和STAT 3蛋白的表达水平。结果与NC组比较,SAP各组AMY水平均明显升高（P〈0.01）;光镜下胰腺和肺组织损伤随病情进展而逐渐加重;SAP组肺湿/干重比与NC组比较显...

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Bibliographic Details
Published in解放军医学杂志 Vol. 36; no. 6; pp. 611 - 613
Main Author 李敏利 朱人敏 张晓华 郭婧芸 杨妙芳 吴晓尉 郭美霞
Format Journal Article
LanguageChinese
Published 2011
Subjects
信号传导
急性胰腺炎
细胞因子类
肺损伤
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Summary:目的探讨JAK2/STAT 3信号通路在实验性重症急性胰腺炎（SAP）肺损伤中的作用。方法以4%牛磺胆酸钠胰胆管逆行注射诱导大鼠SAP模型。32只雄性SD大鼠随机分为4组：对照组（NC组）和SAP 6h、12h、18h组,每组8只。动态测定各组血清淀粉酶（AMY）水平;光镜下观察胰腺及肺组织病理变化,并计算肺湿/干重比;ELISA法检测血清IL-6及IL-18表达情况;West-ern blotting检测肺组织中JAK2和STAT 3蛋白的表达水平。结果与NC组比较,SAP各组AMY水平均明显升高（P〈0.01）;光镜下胰腺和肺组织损伤随病情进展而逐渐加重;SAP组肺湿/干重比与NC组比较显著升高（P〈0.01）。各组血清中均有IL-6及IL-18表达,与NC组比较,SAP各组IL-6及IL-18表达水平均显著上调（P〈0.01）。NC组有极少量的JAK2和STAT 3表达,SAP各组JAK2和STAT 3蛋白表达明显高于NC组,且随造模时间延长逐渐增高（P〈0.01）;JAK2和STAT 3表达变化与肺组织的严重程度一致。结论 JAK2/STAT 3信号通路的激活可诱导IL-6及IL-18过度表达,可能加重SAP时的炎症反应和肺损伤。
Bibliography:LI Min-li,ZHU Ren-min,ZHANG Xiao-hua,GUO Jing-yun,YANG Miao-fang,WU Xiao-wei,GUO Mei-xia.Department of Gastroenterology,Nanjing General Hospital of Nanjing Command,Nanjing 210002,China
11-1056/R
Objective To investigate the mechanism of action of JAK/STAT signaling pathways in the lung injury of experimental severe acute pancreatitis（SAP）.Methods The rat model of SAP was reproduced by retrograde injection of 4% sodium taurocholate into the biliopancreatic duct.Thirty-two male SD rats were randomly assigned into 4 groups（8 each）： normal control group（NC）,SAP 6h,12h and 18h groups.The level of serum amylase（AMY） was measured dynamically.The pathological changes in pancreas and lung were observed under the light microscope,and the wet/dry weight ratios of lung were evaluated.The concentrations of IL-6 and IL-18 were determined by ELISA,and the expressions of JAK2 and STAT3 protein in lung were determined by Western blotting.Results Compared with NC group,serum level of AMY increased significantly in SAP groups（P0.
ISSN:0577-7402