SUPPRESSOR OF VARIEGATION4, a New var2 Suppressor Locus, Encodes a Pioneer Protein that Is Required for Chloroplast Biogenesis

• Published in: 分子植物 Vol. 4; no. 2; pp. 229 - 240
• Main Author: Fei Yu ung-Soon Park Xiayan Liu Andrew Foudree Aigen Fu Marta Powikrowska Anastassia Khrouchtchova Poul Erik Jensen Jillian N. Kriger Gordon RI Gray Steven R. Rodermel
• Format: Journal Article
• Language: Chinese
• Published: 2011
• Subjects: 叶绿体; 基因突变; 生源; 类囊体膜蛋白; 编码; 蛋白质; 蛋白酶类; 非光化学猝灭
• ISSN: 1674-2052; 1752-9867

VAR2 is an integral thylakoid membrane protein and a member of the versatile FtsH class of metalloproteases in prokaryotes and eukaryotes. Recessive mutations in the VAR2 locus give rise to variegated plants （var2） that contain white sectors with abnormal plastids and green sectors with normal-appearing chloroplasts. In a continuing effort to isolate second-site suppressors of var2 variegation, we characterize in this report ems2505, a suppressor strain that has a virescent phenotype due to a missense mutation in At4g28590, the gene for a pioneer protein. We designated this gene SVR4 （for SUPPRESSOR OF VARIEGATION4） and the mutant allele in ems2505 as svr4-1. We demonstrate that SVR4 is located in chloroplasts and that svr4-1 single mutants are normal with respect to chloroplast anatomy and thylakoid membrane protein accumulation. However, they are modestly impaired in several aspects of photochemistry and have enhanced non-photochemical quenching （NPQ） capacity. A T-DNA insertion allele of SVR4, svr4-2, is s