Design of a multiplex PCR method for detection of toxigenicpathogenic in Vibrio cholerae

• Published in: 亚太热带医药杂志：英文版 no. 2; pp. 115 - 118
• Main Author: Abbas Ali Imani Fooladi Iman Islamieh D Hosseini Doust R Karami A Marashi SM
• Format: Journal Article
• Language: English
• Published: 2013
• Subjects: cholerae; detection; Multiplex; PCR; Rapid; Vibrio
• ISSN: 1995-7645; 2352-4146

<正>Objective:To study virulence and regulatory genes(hlyA,ctxB,tcpI) in clinical strains of Vibrio ckolerae(V.cholerae),simultaneously.Methods:Three important genes,tepI,hlyA and ctxB were used for detection of toxigenic and pathogenic V.cholera by chain reaction assay method. Results:According to the results of the PCR,the incidence of hlyA,tcpI,and ctxB genes in clinical isolates was obtained as 94.7%(72 sample),90.8%(69 sample),and 92.1%(70 sample), respectively.Five strains possessed all genes except ctxB,six strains possessed all genes except tcpI,four strains possessed all genes except hlyA,one strain possessed only hlyA and 60 strains contained a combination of three genes.Including hlyA,ctxB and tcpI,Conclusions:Result show that this method could be reliable to detect toxigenic-pathogenic strains of V.cholerae in Iran.