Influence of medium components on elastase production using crude sources by Bacillus sp.EL31410

• Published in: Journal of Zhejiang University. Science Vol. 4; no. 2; pp. 142 - 151
• Main Author: 何国庆 陈启和 张丽 刘小杰
• Format: Journal Article
• Language: English
• Published: 2003
• Subjects: 中间成分; 天然原料; 实验设计; 弹性蛋白酶; 杆状细菌; 表面反应方法论
• ISSN: 1009-3095

A newly isolated strain EL31410,producing elastase(E.C3.4.4.7) with high elastolytic activity was identified as Bacillus sp.In the medium optimization,it was found that wheat bran and soybean flour hydrosate were the best crude carbon ad nitrogen source for enzyme production,respectively.Addition of com steep flour can affect the bacterium growth and elastase production.A fractional factorial design was ap-plied to study the main factors that affect the enzyme production,and central composite experimental design and response surface methodology were adopted to derive a statistical model for the effect of wheat bran and soybean flour hydrosate on elastase production.The experimental results showed that wheat bran had positive cffect but soybean flour hydrosate had negative effect,on enzyme production.An initial concentration of 3.4%(w/v) wheat bran and 9.4%（v/v) soybean flour hydrosate were found to be optimal for enzyme produc-tion in batch culture.The time course of elastase production in the optimized medium composition was also de-scribed.