Identification and Characterization of a New IgE-binding Protein in Mackerel （Scomberjaponicus） by MALDI-TOF-MS

• Published in: Journal of Ocean University of China Vol. 10; no. 1; pp. 93 - 98
• Main Author: WANG Bangping LI Zhenxing ZHENG Lina LIU Yixuan LIN Hong
• Format: Journal Article
• Language: English
• Published: 2011
• Subjects: MALDI; TOF; 基质辅助激光解吸; 电离飞行时间质谱; 磷酸丙糖异构酶; 结合蛋白; 食物过敏原
• ISSN: 1672-5182; 1993-5021

As fish is one source of the ‘big eight’ food allergens,the prevalence of fish allergy has increased over the past few years.In order to better understand fish allergy,it is necessary to identify fish allergens.Based on the sera from fish-allergenic patients,a 28 kDa protein from local mackerel （Scomber japonicus）,which has not been reported as a fish allergen,was found to be reactive with most of the patients’ sera.The 28 kDa protein was analyzed by MALDI-TOF-MS （Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry）.Mascot search in NCBI database （Date： 08/07/2010） showed that the top protein matched,i.e.triosephosphate isomerase （TPI） from Xiphophorus maculatus and Poecilia reticulata,had a mowse （molecular weight search） score of 98.In addition,TPI from Epinephelus coioides also matched this mackerel protein with a mowse score of 96.Because TPI is con-sidered as an allergen in other non-fish organisms,such as lychee,wheat,latex,archaeopotamobius （Archaeopotamobius sibiriensis） and crangon （Crangon crangon）,we consider that it may also be an allergen in mackerel.