核苷中2'-位取代基对Burkholderia cepacia脂肪酶选择性酰化核苷的影响

研究了核苷结构中2'-位含有不同取代基时Burkholderia cepacia脂肪酶促3'-位羟基高区域选择性酰化反应.结果表明,该脂肪酶催化核苷类似物酰化的优势产物均为3'-酯,且随着酰基供体链的增长,3'-区域选择性逐渐升高;在酶促核苷癸酰化反应中,当核苷2'-位含有H,F,Cl和Br取代基时,3'-区域选择性最高(89%~98%);当2'-位含有OH或OCH3时,3'-区域选择性较低(69%~74%)....

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Bibliographic Details
Published inChinese journal of catalysis Vol. 32; no. 2; pp. 357 - 361
Main Author 王朝宇 宗敏华
Format Journal Article
LanguageChinese
Published 2011
Subjects
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Summary:研究了核苷结构中2'-位含有不同取代基时Burkholderia cepacia脂肪酶促3'-位羟基高区域选择性酰化反应.结果表明,该脂肪酶催化核苷类似物酰化的优势产物均为3'-酯,且随着酰基供体链的增长,3'-区域选择性逐渐升高;在酶促核苷癸酰化反应中,当核苷2'-位含有H,F,Cl和Br取代基时,3'-区域选择性最高(89%~98%);当2'-位含有OH或OCH3时,3'-区域选择性较低(69%~74%).
Bibliography:nucleoside analog; regioselective acylation; enzyme catalysis; substrate recognition
O643
regioselective acylation
enzyme catalysis
nucleoside analog
substrate recognition
21-1195/O6
ISSN:0253-9837
1872-2067