Transcript and Sequence Analysis of S-RNases in Self-Compatible Tetraploid Chinese Cherry （Prunus pseudocerasus L.）

• Published in: Agricultural sciences in China no. 6; pp. 792 - 798
• Main Author: ZHANG Xiao-yan CHEN Xue-sen MENG Qing-wei JIANG Yuan-mao ZHENG Yang CHEN Xiao-liu
• Format: Journal Article
• Language: English
• Published: 2010
• Subjects: Northern杂交; RNase; 中国樱桃; 全长cDNA序列; 基因转录; 异源四倍体; 核糖核酸酶
• ISSN: 1671-2927

Chinese cherry （Prunus pseudocerasus L.） is an allotetraploid species and exhibits natural self-compatibility.However,the full-length cDNA sequences,functional analysis and the transcripts of S-RNase alleles in Chinese cherry cultivars are not known.In the two cultivars Taixiaohongying and Laiyang Short Cherry with S1S2S3S4 genotypes,two S-RNases were transcribed in Northern blotting,and the two full-length cDNAs of S-RNase were cloned and analyzed.As the result,the transcribed S-RNases were S1-RNase and S2-RNase.The two complete cDNA sequences of S1-RNase and S2-RNase were registered as EU073938 and EU073939,respectively,and had characteristic structure of rosaceous S-RNases based on their sequences indicating that they had normal function for S-RNase in the style.The S3-RNase and S4-RNase were not transcribed in the style and were nonfunctional for S-RNase,so S3m and S4m could be used to represent the nonfunctional S3-RNase and S4-RNase.The phylogenetic analysis implied that the S-RNases of Prunus,including Chinese cherry,had lower intra-specific similarity and diverged earlier than the divergence of species in Prunus.