Organ-Specific Responses in the Shoots and Roots of Suaeda salsa to NaCI of Vacuolar H^＋-ATPase C3 Halophyte

• Published in: Journal of integrative plant biology no. 3; pp. 308 - 314
• Main Author: Ming-Feng Yang Jie Song Bao-ShanWang
• Format: Journal Article
• Language: English
• Published: 2010
• Subjects: ATP酶; NaCl溶液; 器官特异性; 牛血清蛋白; 盐地碱蓬; 盐生植物
• ISSN: 1672-9072; 1744-7909

Suaeda salsa L. is a halophytic species that is well adapted to high salinity. In order to understand its salt tolerance mechanism, we examined the growth and vacuolar H^＋-ATPase （V-ATPase） response to NaCI within the shoots and roots. The growth of shoots, but not roots, was dramatically stimulated by NaCI. CI^- and Na^＋ were mainly accumulated in shoots. V-ATPase activity was significantly increased by NaCI in roots and especially in shoots. Interestingly, antisera ATP95 and ATP88b detected three V1 subunits （66, 55 and 36 KDa） of V-ATPase only in shoots, while an 18 kDa V0 subunit of V-ATPase was detected by both antisera in shoots and roots. It suggested that the tissue-specific characteristics of V-ATPase were related to the different patterns of growth and ion accumulation in shoots and roots of S. salsa.