Genetic analysis and molecular mapping of a novel gene for zebra mutation in rice （Oryza sativa L.）

• Published in: Journal of genetics and genomics no. 11; pp. 679 - 684
• Main Author: Qiushi Wang Xianchun Sang Yinghua Ling Fangming Zhao Zhenglin Yang Yunfeng Li Guanghua He
• Format: Journal Article
• Language: Chinese
• Published: 2009
• Subjects: SSR标记; 叶绿素含量; 斑马; 新基因; 映射; 水稻; 突变体; 遗传分析
• ISSN: 1673-8527

A novel zebra mutant, zebra-15, derived from the restorer line JinhuilO （Oryza sativa L. ssp. indica） treated by EMS, displayed a distinctive zebra leaf from seedling stage to jointing stage. Its chlorophyll content decreased （55.4%） and the ratio of Chla/Chlb increased （90.2%） significantly in the yellow part of the zebra-15, compared with the wild type. Net photosynthetic rate and fluorescence kinetic parameters showed that the decrease of chlorophyll content significantly influenced the photosynthetic efficiency of the mutant. Genetic analysis of F2 segregation populations derived from the cross of XinonglA and zebra-15 indicated that the zebra leaf trait is controlled by a single recessive nuclear gene. Ninety-eight out of four hundred and eighty pairs of SSR markers showed the diversity between the XinonglA and the zebra-15, their F2 population was then used for gene mapping. Zebra-15 （Z-15） gene was primarily restricted on the short arm of chromosome 5 by 150 F2 recessive individuals, 19.6 cM from marke