Cotton GhPOX1 encoding plant class III peroxidase may be responsible for the high level of reactive oxygen species production that is related to cotton fiber elongation

• Published in: Journal of genetics and genomics no. 3; pp. 141 - 150
• Main Author: Wenqian Mei Yongmei Qin Wenqiang Song Jun Li Yuxian Zhu
• Format: Journal Article
• Language: Chinese
• Published: 2009
• Subjects: 棉; 植物细胞; 纤维特性; 过氧化物酶
• ISSN: 1673-8527

The accumulation of reactive oxygen species （ROS） is involved in plant cell development. In plant, class III peroxidases are heme-containing enzymes encoded by a large multi-gene family participated in the release or consumption of ROS. The specific function of each member of the family is still elusive. Here, we showed that ROS was significantly generated during cotton fiber initiation and elongation, whereas, application of NADPH oxidase inhibitor diphenyleneiodonium （DPI） and peroxidase inhibitor salicylhydroxamic acid （SHAM） to the wild-type cotton ovule culture significantly suppressed fiber growth, respectively. Their inhibitory effects were caused by the reduction of superoxide radical （O2^-）. Ten GhPOX genes （cDNAs） encoding cotton class III peroxidases were isolated, among them eight GhPOX genes were reported for the first time. Microarray analyses indicated that GhPOX1 was the mostly predominantly expressed in fast-elongating cotton fiber cells. Real-time quantitative PCR analysis revealed the trans