Screening of Extraction Methods for Glycoproteins from Jellyfish （Rhopilema esculentum） Oral-Arms by High Performance Liquid Chromatography

• Published in: Journal of Ocean University of China Vol. 8; no. 1; pp. 83 - 88
• Main Author: REN Guoyan LI Bafang ZHAO Xue ZHUANG Yongliang YAN Mingyan HOU Hu ZHANG Xiukun CHEN Li
• Format: Journal Article
• Language: English
• Published: 2009
• Subjects: HPLC; 糖蛋白; 萃取法; 高性能液体色谱
• ISSN: 1672-5182; 1993-5021

In order to select an optimum extraction method for the target glycoprotein （TGP） from jellyfish （Rhopilema esculentum） oral-arms, a high performance liquid chromatography （HPLC）-assay for the determination of the TGP was developed Purified target glycoprotein was taken as a standard glycoprotein. The results showed that the calibration curves for peak area plotted against concentration for TGP were linear （r= 0.9984, y=4.5895x＋47.601） over concentrations ranging from 50 to 400mgL^-1. The mean extraction recovery was 97.84% （CV2.60%）. The fractions containing TGP were isolated from jellyfish （R esculentum） oral-arms by four extraction methods： 1） water extraction （WE）, 2） phosphate buffer solution （PBS） extraction （PE）, 3） ultrasound-assisted water extraction （UA-WE）, 4） ultrasound-assisted P/3S extraction （UA-PE）. The lyophilized extract was dissolved in Milli-Q water and analyzed directly on a short TSK-GEL G4000PWXL （7.8 mm×300 ram） column. Our results indicated that the UA-PE method was the optimum extraction method selected by HPLC.