Correlation of genomic and expression alterations of AS3 with esophageal squamous cell carcinoma

• Published in: Journal of genetics and genomics Vol. 35; no. 5; pp. 267 - 271
• Main Author: Yu Zhang Xiaoping Huang Jun Qi Cai Yan Xin Xu Yaling Han Mingrong Wang
• Format: Journal Article
• Language: Chinese
• Published: 2008
• Subjects: 基因表达; 异型接合性; 激素; 食管鳞细胞癌
• ISSN: 1673-8527

Androgen-induced proliferation shutoff gene AS3, also known as APRIN, is a growth inhibitory gene that is initially implicated in prostate cancer. This gene is required for androgen-dependent growth arrest and is a primary target for 1,25（OH）2D3 and androgens. Allelic loss at AS3 locus has been linked to a variety of cancers. However, the correlation of genomic and expression alterations of AS3 with esophageal squamous cell carcinoma （ESCC） is not well established. In this study, the genornic and expression alterations of AS3 in ESCC and their clinical significance are evaluated. Loss of heterozygosity （LOH） analysis using an AS3 intragenic microsatellite marker D13S171 revealed 72% allelic loss at AS3 locus in ESCC, which is significantly correlated with higher pathological grade （P=0.042）. RT-PCR examination showed that AS3 mRNA obviously decreased in 44% tumors and its down-regulation was correlated with the sex of patients （P=0.03）. Furthermore, the correlation between genornic and expression alterations