Cloning and Expression of BTB/POZ-Domain from Zebrafish gcl （Germ Cell-Less）, and Appraisement of Its Polyclonal-Antibody
In this study, a recombinant pET28c-gBTB/POZ was constructed by cloning the sequence of the BTB/POZ domain of the zebrafish gcl （germ cell-less） into the expression vector pET28c, and pET28c-gBTB/POZ was transformed into BL21（DE3） pLysS strain to express the fusion protein for the preparation of ant...
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| Published in | Wuhan University journal of natural sciences Vol. 13; no. 1; pp. 123 - 128 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
2008
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| Subjects | |
| Online Access | Get full text |
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| Abstract | In this study, a recombinant pET28c-gBTB/POZ was constructed by cloning the sequence of the BTB/POZ domain of the zebrafish gcl （germ cell-less） into the expression vector pET28c, and pET28c-gBTB/POZ was transformed into BL21（DE3） pLysS strain to express the fusion protein for the preparation of antibody. Polyclonal-antibody against the GCL-BTB/POZ domain was prepared by immunizing rabbit with the fusion protein, and the Western Blot and immuno-histochemical analysis were performed to detect the quantity of the polyclonal-antibody. The result indicates that the polyclonal-antibodies were of good quantity and specification. Further studies will be performed to demonstrate the function and expression pattern of the GCL protein during the development process of zebrafish with the polyclonal-antibody. |
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| AbstractList | In this study, a recombinant pET28c-gBTB/POZ was constructed by cloning the sequence of the BTB/POZ domain of the zebrafish gcl （germ cell-less） into the expression vector pET28c, and pET28c-gBTB/POZ was transformed into BL21（DE3） pLysS strain to express the fusion protein for the preparation of antibody. Polyclonal-antibody against the GCL-BTB/POZ domain was prepared by immunizing rabbit with the fusion protein, and the Western Blot and immuno-histochemical analysis were performed to detect the quantity of the polyclonal-antibody. The result indicates that the polyclonal-antibodies were of good quantity and specification. Further studies will be performed to demonstrate the function and expression pattern of the GCL protein during the development process of zebrafish with the polyclonal-antibody. |
| Author | DENG Fengjiao CHENG Pengxiang LAN Qiang XU Yan ZHUANG Tiangang |
| AuthorAffiliation | College of Life Sciences, Wuhan University / Key Laboratory of the Ministry of Education for Developmental Biology, Wuhan 430072, Hubei, China Center for Research on Reproduction and Women＇s Health, University of Pennsylvania Medical Center, Philadelphia, PA 19104-6080, USA |
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| DocumentTitleAlternate | Cloning and Expression of BTB/POZ-Domain from Zebrafish gcl （Germ Cell-Less）, and Appraisement of Its Polyclonal-Antibody |
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| Notes | gel （germ cell-less）; polycolonal-antibody; BTB/ POZ domain; zebrafish （Danio rerio） BTB/ POZ domain polycolonal-antibody zebrafish （Danio rerio） Q344 42-1405/N gel （germ cell-less） |
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| PublicationYear | 2008 |
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| Snippet | In this study, a recombinant pET28c-gBTB/POZ was constructed by cloning the sequence of the BTB/POZ domain of the zebrafish gcl （germ cell-less） into the... |
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| StartPage | 123 |
| SubjectTerms | 克隆技术 发生遗传学 多克隆技术 生物化学 |
| Title | Cloning and Expression of BTB/POZ-Domain from Zebrafish gcl （Germ Cell-Less）, and Appraisement of Its Polyclonal-Antibody |
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