Cloning and Expression of BTB/POZ-Domain from Zebrafish gcl （Germ Cell-Less）, and Appraisement of Its Polyclonal-Antibody

In this study, a recombinant pET28c-gBTB/POZ was constructed by cloning the sequence of the BTB/POZ domain of the zebrafish gcl （germ cell-less） into the expression vector pET28c, and pET28c-gBTB/POZ was transformed into BL21（DE3） pLysS strain to express the fusion protein for the preparation of ant...

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Bibliographic Details
Published inWuhan University journal of natural sciences Vol. 13; no. 1; pp. 123 - 128
Main Author DENG Fengjiao CHENG Pengxiang LAN Qiang XU Yan ZHUANG Tiangang
Format Journal Article
LanguageEnglish
Published 2008
Subjects
克隆技术
发生遗传学
多克隆技术
生物化学
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Summary:In this study, a recombinant pET28c-gBTB/POZ was constructed by cloning the sequence of the BTB/POZ domain of the zebrafish gcl （germ cell-less） into the expression vector pET28c, and pET28c-gBTB/POZ was transformed into BL21（DE3） pLysS strain to express the fusion protein for the preparation of antibody. Polyclonal-antibody against the GCL-BTB/POZ domain was prepared by immunizing rabbit with the fusion protein, and the Western Blot and immuno-histochemical analysis were performed to detect the quantity of the polyclonal-antibody. The result indicates that the polyclonal-antibodies were of good quantity and specification. Further studies will be performed to demonstrate the function and expression pattern of the GCL protein during the development process of zebrafish with the polyclonal-antibody.
Bibliography:gel （germ cell-less）; polycolonal-antibody; BTB/ POZ domain; zebrafish （Danio rerio）
BTB/ POZ domain
polycolonal-antibody
zebrafish （Danio rerio）
Q344
42-1405/N
gel （germ cell-less）
ISSN:1007-1202
1993-4998