Study on the specific interaction between angiogenin and aptamer by atomic force microscopy （AFM）

The specific interaction between angiogenin and aptamer has been investigated by using AFM. The specificity of the interaction is revealed by comparing the binding probability of aptamer to other elements in a series of control experiments. The results have shown that there is specific interaction f...

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Bibliographic Details
Published inChinese science bulletin Vol. 53; no. 2; pp. 198 - 203
Main Author HE XiaoXiao JIN Rong YANG Liu WANG KeMin LI Wei TAN WeiHong LI HuiMin
Format Journal Article
LanguageEnglish
Published 2008
Subjects
交互作用
力检测
原子力显微术
血管生成素
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Summary:The specific interaction between angiogenin and aptamer has been investigated by using AFM. The specificity of the interaction is revealed by comparing the binding probability of aptamer to other elements in a series of control experiments. The results have shown that there is specific interaction force between angiogenin and aptamer. Moreover, the single molecular pull-off force between angiogenin and aptamer has also been determined using the Poisson statistical method to be 133.7±11.7 pN. These findings obtained are helpful to the better revelation of recognition mechanism between angiogenin and aptamer, which provided basis for further understanding the inhibition of the aptamer to angiogenic activity.
Bibliography:R4
11-1785/N
atomic force microscopy （AFM）, angiogenin, aptamer, force detection
ISSN:1001-6538
1861-9541