Application of quantitative PCR method in detection of Lymphocystis disease virus China （LCDV-cn） in Japanese flounder （Paralichthys olivaceus）

Lymphocystis disease causes serious economic losses in the fish farming industry. The causative agent of the disease is Lymphocystis disease virus China （LCDV-cn）, which has a wide range of hosts. Based on competitive quantitative PCR technology, we established a method to quantify the LCDV-cn in ti...

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Bibliographic Details
Published inChinese journal of oceanology and limnology Vol. 25; no. 4; pp. 418 - 422
Main Author 昝金东 孙修勤 张之文 曲凌云 张进兴
Format Journal Article
LanguageEnglish
Published 2007
Subjects
淋巴疾病
病毒数量
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ISSN0254-4059
1993-5005

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Summary:Lymphocystis disease causes serious economic losses in the fish farming industry. The causative agent of the disease is Lymphocystis disease virus China （LCDV-cn）, which has a wide range of hosts. Based on competitive quantitative PCR technology, we established a method to quantify the LCDV-cn in tissue. Results demonstrate that the average amount of LCDV-cn in the peripheral blood of infected flounder with evident tumors is about 10^6virions/ml while the average amount in those flounder with no evident tumor but cultured with the flounder with evident tumor is about 10^4virions/ml. No virus was found in the negative samples of flounder.
Bibliography:Lymphocystis Disease
S941
37-1150/P
Virus Amount
Lymphocystis Disease; Quantitative PCR; Virus Amount
Quantitative PCR
ISSN:0254-4059
1993-5005