Proliferation and differentiation into endothelial cells of human bone marrow mesenchymal stem cells （MSCs） on poly DL-lactic-co-glycolic acid （PLGA） films

• Published in: Chinese science bulletin Vol. 51; no. 11; pp. 1328 - 1333
• Main Author: YUE Huimin ZHANG Lei WANG Yunfang LIANG Feng GUAN Lidong Li Shaoqing YAN Fang NAN Xue BAI Cixian LIN Feng YAN Yongnian PEI Xuetao
• Format: Journal Article
• Language: English
• Published: 2006
• Subjects: MSCs; PLGA; 内皮化; 内皮细胞; 维管组织工程
• ISSN: 1001-6538; 1861-9541

The functional realization is the most important problem in vascular tissue engineering. The small-caliber blood vessel substitutes are prone to thrombi, which results in functional loss of blood vessels. However, this is probably due to the imperfection of endothelial layer in the substitutes. In this study, MSCs were seeded on a series of porous PLGA films with various porosity and pore size made by sodium chloride （NaCI） particulate leaching, and cell proliferation on each film was inspected. The film made of the 75% （w/w） particulate proportion and 30--50μm pore size maximized the proliferation rate and was chosen as the scaffolds for the differentiation of MSCs into endothelial cells. The induced cells expressed endothelial cells specific FIk-1, W factor and CD34, possessed endothelial cells specific Weible-palade （W-P） body, and had the abilities of ingesting low density lipoprotein and secreting prostacyclin （PGI2）. The results show that MSCs not only have the ideal biological compatibility with the porous PLGA films, but also have the potency of differentiating into functional endothelial cells, which should facilitate the endothelialization in vascular tissue engineering.