A STUDY ON THE EXTRACTION PROCESS OF ACTIVE INGREDIENTS FROM AKEBIA STEM AND AN ANALYSIS OF THEIR ANTI-GASTRIC CANCER ACTIVITY
The study investigated the extraction process of active ingredients from akebia stem and an analysis of their anti-gastric cancer activity. Three different extraction methods were used to obtain extracts, namely the decoction method (group A), reflux extraction method (group B), and maceration metho...
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Published in | African journal of traditional, complementary, and alternative medicines Vol. 10; no. 5 |
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Main Authors | , , , , , |
Format | Journal Article |
Published |
Nigeria
African Ethnomedicines Network
14.07.2014
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Subjects | |
Online Access | Get full text |
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Summary: | The study investigated the extraction process of active ingredients
from akebia stem and an analysis of their anti-gastric cancer activity.
Three different extraction methods were used to obtain extracts, namely
the decoction method (group A), reflux extraction method (group B), and
maceration method (group C), of which reflux extraction method and
maceration method used ethanol as the extraction solvent, while
decoction method used distilled water for extraction. The differences
in anti-gastric cancer activity of the three extracts were compared.
MTT assay was used to test and compare the inhibitory effects of
extracts obtained in A, B, and C groups on gastric cancer cells. The
results showed that the dry extract obtained by heat reflux extraction
with "water-ethanol" ratio of 1:2, extractant volume of 70
ml, with ethanol as extraction solvent presented the best inhibitory
activity on gastric cancer SGC-7901 cells in this study. Its inhibitory
effect did not change over time, and was directly proportional to the
concentration. |
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ISSN: | 0189-6016 2505-0044 |