Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni

The immunoreactivity of seven peptides synthesized from Schistosoma mansoni proteins, was evaluated by dot-blot and ELISA assays using two different sensitization methodologies. The best results were obtained on wells of the Costar 3590 microplates coated with peptides P1, P2, P3, P6, and P7 using c...

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Published inMemórias do Instituto Oswaldo Cruz Vol. 101; no. s1
Main Authors Edward José de Oliveira, Hermínia Yohko Kanamura, Kioko Takei, Rosario Dominguez Crespo Hirata, Nga Yen Nguyen, Mário Hiroyuki Hirata
Format Journal Article
Published Brazil Fundação Oswaldo Cruz, Fiocruz 11.01.2007
Subjects
immunodiagnosis
Schistosoma mansoni
synthetic peptides
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Summary:The immunoreactivity of seven peptides synthesized from Schistosoma mansoni proteins, was evaluated by dot-blot and ELISA assays using two different sensitization methodologies. The best results were obtained on wells of the Costar 3590 microplates coated with peptides P1, P2, P3, P6, and P7 using conventional methodology. The signals increased considerably (p < 0.0003) on wells sensitized with P1 to P6 using alternative methodology. In contrast, the well coated with peptide P7 presented lower signal when compared with conventional methodology (p = 0.0019). These results, establish the basis for the application of synthetic peptides for laboratory diagnosis of schistosomiasis mansoni.
ISSN:1678-8060
1678-8060