Application of synthetic peptides in development of a serologic method for laboratory diagnosis of schistosomiasis mansoni
The immunoreactivity of seven peptides synthesized from Schistosoma mansoni proteins, was evaluated by dot-blot and ELISA assays using two different sensitization methodologies. The best results were obtained on wells of the Costar 3590 microplates coated with peptides P1, P2, P3, P6, and P7 using c...
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Published in | Memórias do Instituto Oswaldo Cruz Vol. 101; no. s1 |
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Main Authors | , , , , , |
Format | Journal Article |
Published |
Brazil
Fundação Oswaldo Cruz, Fiocruz
11.01.2007
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Subjects | |
Online Access | Get full text |
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Summary: | The immunoreactivity of seven peptides synthesized from Schistosoma
mansoni proteins, was evaluated by dot-blot and ELISA assays using two
different sensitization methodologies. The best results were obtained
on wells of the Costar 3590 microplates coated with peptides P1, P2,
P3, P6, and P7 using conventional methodology. The signals increased
considerably (p < 0.0003) on wells sensitized with P1 to P6 using
alternative methodology. In contrast, the well coated with peptide P7
presented lower signal when compared with conventional methodology (p =
0.0019). These results, establish the basis for the application of
synthetic peptides for laboratory diagnosis of schistosomiasis mansoni. |
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ISSN: | 1678-8060 1678-8060 |