Bioconversion and saccharification of some lignocellulosic wastes by Aspergillus oryzae ITCC-4857.01 for fermentable sugar production
The recent interest in bioconversion of agricultural and industrial wastes to chemical feedstock has led to extensive studies on cellulolytic enzymes produced by microorganisms. In the present study three lignocellulosic substrates viz. sugarcane bagasse, sawdust and water hyacinth were pre-treated...
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Published in | Electronic Journal of Biotechnology Vol. 14; no. 5 |
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Main Authors | , |
Format | Journal Article |
Published |
Chile
Universidad Católica de Valparaíso
18.10.2012
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Subjects | |
Online Access | Get full text |
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Summary: | The recent interest in bioconversion of agricultural and industrial
wastes to chemical feedstock has led to extensive studies on
cellulolytic enzymes produced by microorganisms. In the present study
three lignocellulosic substrates viz. sugarcane bagasse, sawdust and
water hyacinth were pre-treated with alkali and enzyme and their effect
on bioconversion has been investigated. The ability of selected
substrates for induction of cellulase enzyme by A. oryzae ITCC
4857.01 and for the potentiality of the induced enzyme to saccharify
the substrates were also assessed. The maximum degree of conversion of
substrate (0.415%) and improved specific substrate consumption (0.99 g
substrate/g dry biomass) was exhibited in sugarcane bagasse after
alkali treatment at 96 hrs. Both alkali-treatment and enzyme-treatment,
water hyacinth was the best for cellulase induction and showed maximum
endoglucanase activity of 11.42 U/ml. Reducing sugar yield ranged from
1.12 mg/ml for enzyme treated sawdust at 48 hrs to 7.53 mg/ml for
alkali treated sugarcane bagasse at 96 hrs. Alkali-treated sugarcane
bagasse gave the highest saccharification rate of 9.03% after 96 hrs.
The most resistant substrate was sawdust which produced 5.92%
saccharification by alkaline treatment. The saccharification of
lignocellulosic substrates by enzyme produced by A. oryzae ITCC 4857.01
indicates the enzymes specificity towards the substrates. The use of
such enzyme in lingo-cellulose hydrolysis will lead to efficient
conversion of cellulose materials to other important products. |
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ISSN: | 0717-3458 0717-3458 |