Quantum-Enhanced Detection of Viral cDNA via Luminescence Resonance Energy Transfer Using Upconversion and Gold Nanoparticles
The COVID-19 pandemic has profoundly impacted global economies and healthcare systems, revealing critical vulnerabilities in both. In response, our study introduces a groundbreaking method for the detection of SARS-CoV-2 cDNA, leveraging Luminescence resonance energy transfer (LRET) between upconver...
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Main Authors | , , , , , , , , , , , , |
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Format | Journal Article |
Language | English |
Published |
13.10.2024
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Subjects | |
Online Access | Get full text |
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Summary: | The COVID-19 pandemic has profoundly impacted global economies and healthcare
systems, revealing critical vulnerabilities in both. In response, our study
introduces a groundbreaking method for the detection of SARS-CoV-2 cDNA,
leveraging Luminescence resonance energy transfer (LRET) between upconversion
nanoparticles (UCNPs) and gold nanoparticles (AuNPs) to achieve an
unprecedented detection limit of 242 femtomolar (fM). This innovative sensing
platform utilizes UCNPs conjugated with one primer and AuNPs with another,
targeting the 5' and 3' ends of the SARS-CoV-2 cDNA, respectively, enabling
precise differentiation of mismatched DNA sequences and significantly enhancing
detection specificity. Through rigorous experimental analysis, we established a
quenching efficiency range from 10.4\% to 73.6\%, with an optimal midpoint of
42\%, thereby demonstrating the superior sensitivity of our method. By
comparing the quenching efficiency of mismatched DNAs to the target DNA, we
identified an optimal DNA:UCNP:AuNP ratio that ensures accurate detection. Our
comparative analysis with existing SARS-CoV-2 detection methods revealed that
our approach not only provides a lower detection limit but also offers higher
specificity and potential for rapid, on-site testing. This study demonstrates
the superior sensitivity and specificity of using UCNPs and AuNPs for
SARS-CoV-2 cDNA detection, offering a significant advancement in rapid,
accessible diagnostic technologies. Our method, characterized by its low
detection limit and high precision, represents a critical step forward in
managing current and future viral outbreaks, contributing to the enhancement of
global healthcare responsiveness and infectious disease control. |
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DOI: | 10.48550/arxiv.2410.10911 |