Transverse Aortic Constriction: a Model to Study Heart Failure in Small Animals

Limitations of in vitro and chemical (angiotensin II or isoproterenol) infusion systems to mimic cardiac hypertrophy and heart failure conditions necessitate a relevant in vivo physiological model to mimic this disease. The leading strategy to develop hypertrophic heart failure in mice is by constri...

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Bibliographic Details
Published inManual of Research Techniques in Cardiovascular Medicine pp. 164 - 169
Main Authors Verma, Suresh Kumar, Krishnamurthy, Prasanna, Kishore, Raj
Format Book Chapter
LanguageEnglish
Published Oxford, UK John Wiley & Sons, Ltd 21.02.2014
Subjects
cardiac hypertrophy
cardiovascular disease
heart failure
surgical procedure
transverse aortic constriction
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Summary:Limitations of in vitro and chemical (angiotensin II or isoproterenol) infusion systems to mimic cardiac hypertrophy and heart failure conditions necessitate a relevant in vivo physiological model to mimic this disease. The leading strategy to develop hypertrophic heart failure in mice is by constriction of the transverse aorta (mimic of aortic stenosis), resulting in excessive load on the left ventricle. Aortic constriction is the most accepted method used by cardiovascular scientists to mimic hypertrophic heart disease. This chapter provides comprehensive details of transverse aortic constriction procedures and will guide the readers through the entire procedure, from the preparation of the animal for surgery until its full recovery; a list of all necessary tools and devices is included. Adhering to our recommendations should improve reproducibility of the model and bring the number of required animal subjects to the minimum.
ISBN:0470672692
9780470672693
DOI:10.1002/9781118495148.ch20