GSH2, a gene encoding γ‐glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha

The GSH2 gene, encoding Hansenula polymorphaγ‐glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)‐deficient gsh2 mutant of H. polymorpha. The gene was isolated as a 4.3‐kb XbaI fragment that was capable of restoring GSH synthesis, heavy‐metal resistance and c...

Full description

Saved in:
Bibliographic Details
Published inFEMS yeast research Vol. 2; no. 3; pp. 327 - 332
Main Authors Ubiyvovk, Vira M, Nazarko, Taras Y, Stasyk, Olena G, Sohn, Min Jeong, Kang, Hyun Ah, Sibirny, Andrei A
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.08.2002
Subjects
Glutathione biosynthesis
Hansenula polymorpha
Methylotrophic yeast
γ‐Glutamylcysteine synthetase
Online AccessGet full text

Cover

More Information
Summary:The GSH2 gene, encoding Hansenula polymorphaγ‐glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)‐deficient gsh2 mutant of H. polymorpha. The gene was isolated as a 4.3‐kb XbaI fragment that was capable of restoring GSH synthesis, heavy‐metal resistance and cell proliferation when introduced into gsh2 mutant cells. It possesses 53% identical and 69% similar amino acids compared with the Candida albicans homologue (Gcs1p). In comparison to the Saccharomyces cerevisiae homologue (Gsh1p), it possesses 47% identical and 61% similar amino acids. The GSH2 sequence appears in the GenBank database under accession No. AF435121.
ISSN:1567-1356
1567-1364
DOI:10.1111/j.1567-1364.2002.tb00101.x