Assessment of Cardiomyocyte Size

Myocytes make up about 75% of ventricular mass but are outnumbered by nonmyocardial cells. Chamber dilatation in progression to heart failure is largely due to myocyte lengthening from series addition of new sarcomeres, while increased cross‐sectional area accounts for changes in wall thickness. Cor...

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Bibliographic Details
Published inManual of Research Techniques in Cardiovascular Medicine pp. 378 - 385
Main Authors Gerdes, A. Martin, Pingitore, Alessandro
Format Book Chapter
LanguageEnglish
Published Oxford, UK John Wiley & Sons, Ltd 21.02.2014
Subjects
collagenase
hypertrophy
left ventricular dysfunction
left ventricular remodeling
morphometry
myocyte
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ISBN0470672692
9780470672693
DOI10.1002/9781118495148.ch44

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Summary:Myocytes make up about 75% of ventricular mass but are outnumbered by nonmyocardial cells. Chamber dilatation in progression to heart failure is largely due to myocyte lengthening from series addition of new sarcomeres, while increased cross‐sectional area accounts for changes in wall thickness. Coronary perfusion with collagenase can be used to obtain isolated myocytes for determination of myocyte dimensions. It consists of the following steps: (1) heart removal and aortic cannulation; (2) coronary perfusion with media followed by collagenase; (3) myocyte dissociation; (4) myocyte fixation; (5) determination of cell volume (V) by Coulter Channelyzer, length (L) microscopically, and cross‐sectional area from V/L. Not only does this approach provide precise, comprehensive, and reproducible results, it allows direct comparisons between all published data collected in this manner.
ISBN:0470672692
9780470672693
DOI:10.1002/9781118495148.ch44