Inositol trisphosphate‐mediated Ca2+ release in beet mu;somes is inhibited by heparin

Microsomes from the storage root of red beet exhibit ATP‐dependent, protonophore‐sensitive Ca2+ accumulation, characteristic of the vacuolar membrane (tonoplast). A portion (20%) of this intravesicular Ca2+ store is specifically released by inositol 1,4,5‐trisphosphate (InsP3) with a K 0.5 = 0.54 μM...

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Bibliographic Details
Published inFEBS letters Vol. 260; no. 1; pp. 70 - 72
Main Authors Brosnan, James M., Sanders, Dale
Format Journal Article
LanguageEnglish
Published 15.01.1990
Subjects
Beta vulgaris
BTP, 1,3-bisltris(hydroxymethyl)methylamino]-propane
Calcium efflux
FCCP, carbonylcyanide p-trifluoromethoxyphenylhydrazone
Heparin
Inositol 1,4,5-trisphosphate
InsP2, inositol 1,4-bisphosphate
InsP3, inositol 1,4,5-trisphosphate
InsP4, inositol 1,3,4,5-tetrakisphosphate
TMB-8, 8-(N,N-diethylamino)-octyl 3,4,5-trimethoxybenzoate-HCl
Tonoplast
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Summary:Microsomes from the storage root of red beet exhibit ATP‐dependent, protonophore‐sensitive Ca2+ accumulation, characteristic of the vacuolar membrane (tonoplast). A portion (20%) of this intravesicular Ca2+ store is specifically released by inositol 1,4,5‐trisphosphate (InsP3) with a K 0.5 = 0.54 μM. InsP3,‐mediated Ca2+ release is eliminated by low concentrations of heparin (K 0.5 = 86 nM). This result highlights the conservation between the animal and plant inositol 1,4,5‐trisphosphate‐mediated Ca2+ release mechanisms, despite the fact that they are located at different intracellular membranes.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(90)80068-T