Multi-wavelength digital holographic microscopy for bio-imaging and applications

A single-shot common-path off-axis multi-wavelength digital holographic microscopic (MW-DHM) system for phase imaging of biological specimens is proposed. The DHM system is based on a cube beam splitter which divides the object beam into two beams: one beam is spatially filtered at its Fourier plane...

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Bibliographic Details
Main Authors Kumar, Manoj, Matoba, Osamu, Quan, Xiangyu, Awatsuji, Yasuhiro
Format Conference Proceeding
LanguageEnglish
Published SPIE 15.06.2020
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Summary:A single-shot common-path off-axis multi-wavelength digital holographic microscopic (MW-DHM) system for phase imaging of biological specimens is proposed. The DHM system is based on a cube beam splitter which divides the object beam into two beams: one beam is spatially filtered at its Fourier plane to make a reference spherical beam and another beam acts as the object beam. Three wavelengths: 473, 532, and 632.8 nm are used to illuminate the object under observation. The obtained experimental results on various objects corroborate the imaging capability of the proposed single-shot common-path off-axis multi-wavelength MW-DHM system. The proposed system may open new possibilities in bio-imaging and real-time simultaneous measurement of various parameters including the thickness, refractive index, etc.
Bibliography:Conference Date: 2020-04-22|2020-04-24
Conference Location: Yokohama, Japan
ISBN:9781510638495
1510638490
ISSN:0277-786X
DOI:10.1117/12.2573229