High Performance Affinity Chromatography of Antithrombin III Based on Monodisperse Poly (glycidyl methacrylate) Beads
O62; A new approach for the separation of antithrombin III with high performance affinity chromatography (HPAC) was described. A novel monodisperse,non-porous,cross-linked poly (glycidyl methacrylate) beads (PGMA) were used as the affinity support. With the water-soluble carbodiimide,heparin was lin...
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Published in | 中国化学快报(英文版) Vol. 12; no. 5; pp. 443 - 446 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Center for Molecular Science, Institute of Chemistry Chinese Academy of Science
01.05.2001
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Online Access | Get full text |
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Abstract | O62; A new approach for the separation of antithrombin III with high performance affinity chromatography (HPAC) was described. A novel monodisperse,non-porous,cross-linked poly (glycidyl methacrylate) beads (PGMA) were used as the affinity support. With the water-soluble carbodiimide,heparin was linked covalently to amino-PGMA-beads,which was prepared by amination of PGMA. The adsorbent obtained exhibits high binding activity to antithrombin III (ATIII),good resolution and excellent mechanical properties and can be used under high flow rate. |
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AbstractList | O62; A new approach for the separation of antithrombin III with high performance affinity chromatography (HPAC) was described. A novel monodisperse,non-porous,cross-linked poly (glycidyl methacrylate) beads (PGMA) were used as the affinity support. With the water-soluble carbodiimide,heparin was linked covalently to amino-PGMA-beads,which was prepared by amination of PGMA. The adsorbent obtained exhibits high binding activity to antithrombin III (ATIII),good resolution and excellent mechanical properties and can be used under high flow rate. |
Author | Guo Quan LIU Rui ZHAO Di Hua SHANGGUAN Tian Sheng SHU Ying Xin ZHAO |
AuthorAffiliation | Center for Molecular Science, Institute of Chemistry Chinese Academy of Science |
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Title | High Performance Affinity Chromatography of Antithrombin III Based on Monodisperse Poly (glycidyl methacrylate) Beads |
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