High Performance Affinity Chromatography of Antithrombin III Based on Monodisperse Poly (glycidyl methacrylate) Beads

O62; A new approach for the separation of antithrombin III with high performance affinity chromatography (HPAC) was described. A novel monodisperse,non-porous,cross-linked poly (glycidyl methacrylate) beads (PGMA) were used as the affinity support. With the water-soluble carbodiimide,heparin was lin...

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Published in中国化学快报(英文版) Vol. 12; no. 5; pp. 443 - 446
Main Authors Ying Xin ZHAO, Di Hua SHANGGUAN, Rui ZHAO, Tian Sheng SHU, Guo Quan LIU
Format Journal Article
LanguageEnglish
Published Center for Molecular Science, Institute of Chemistry Chinese Academy of Science 01.05.2001
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Summary:O62; A new approach for the separation of antithrombin III with high performance affinity chromatography (HPAC) was described. A novel monodisperse,non-porous,cross-linked poly (glycidyl methacrylate) beads (PGMA) were used as the affinity support. With the water-soluble carbodiimide,heparin was linked covalently to amino-PGMA-beads,which was prepared by amination of PGMA. The adsorbent obtained exhibits high binding activity to antithrombin III (ATIII),good resolution and excellent mechanical properties and can be used under high flow rate.
ISSN:1001-8417
1878-5964