Ferrodoxin‐dependent CO2 fixation in bean sprouts

Extracts of bean sprouts are capable of reducing ferrodoxin and of catalyzing the incorporation of bicarbonate and acetyl coenzyme A into an organic compound that is likely to be pyruvate, in a reaction that requires reduced ferredoxin. The rate of the reaction, the first known for which ferredoxin...

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Bibliographic Details
Published inFEBS letters Vol. 359; no. 1; pp. 50 - 52
Main Authors Hirasawa, Masakazu, Koguchi-Kamioka, Osamu, Li, Xin, Knaff, David B.
Format Journal Article
LanguageEnglish
Published 06.02.1995
Subjects
Bean sprout
benzyl isothiocyanate
BITC
CO2 assimilation
EDTA
ethylenediamine tetraacetic acid
Ferredoxin
ferredoxin NADP+ oxidoreductase
FNR
Non-photosynthetic tissue
phenylmethylsulfonyl fluoride
PMSF
Reductive carboxylation
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Summary:Extracts of bean sprouts are capable of reducing ferrodoxin and of catalyzing the incorporation of bicarbonate and acetyl coenzyme A into an organic compound that is likely to be pyruvate, in a reaction that requires reduced ferredoxin. The rate of the reaction, the first known for which ferredoxin appears to serve as the direct reductant for CO2 fixation in a higher plant, depends on the concentrations of both ferredoxin and bicarbonate, with half‐maximal rates being observed at ferredoxin and bicarbonate concentrations of 0.8 μM and 200 μM, respectively.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)00012-X