The simultaneous determination of Quinoline Yellow (E-104) and Sunset Yellow (E-110) in syrups and tablets by second derivative spectrophotometry

• Published in: Turkish journal of chemistry Vol. 26; no. 4; pp. 501 - 508
• Main Authors: KOYUNCU, İkbal, ÖZGÜR, Mahmure, Üstün
• Format: Journal Article
• Language: English
• Published: TÜBİTAK 2002
• Subjects: Analitik Kimya, Ayırma Yöntemleri; Analytical Chemistry, Separation Methods; Binary mixture; Boya; Derivative spectrophotometry; Dye; E-104; E-110; Fizikokimya; food; Gıda; İkili karışım; Kinolin Sarı; Physical Chemistry; Quinoline Yellow; spectrophotometry[analysis]; Spektrofotometri[analiz]; syrup[sugar solution]; Tablet[farmasötikler]; tablet[pharmaceuticals]; Türev spektrofotometrisi; Şeker şerbeti
• ISSN: 1300-0527; 1303-6130

A very simple spectrophotometric method using measurements at zero-crossing wavelength is described for resolving binary mixtures of the food dyes, Sunset Yellow (E-110) and Quinoline Yellow (E-104). Calibration graphs are linear up to 15.0 $\mu gml^{-1}$ of Sunset Yellow (r = 0.9998), and Quinoline Yellow (r = 0.9999). The assay procedure for E-110 and E-104 in pharmaceutical products involves the extraction of the dyes with AcOH/NaOAc buffer solution (pH = 4.5), filtration, and measurement of the second derivative absorbance values at 410.0 nm for E-104 and 533.1 nm for E-110. This method was used for determining synthetic mixtures of these dyes in different ratios and it was successfully applied to two commercial products without a previous separation step.