Using Yeast Two‐Hybrid Methods to Investigate Large Numbers of Binary Protein Interactions

This chapter contains sections titled: Introduction Methods and approaches Troubleshooting References

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Bibliographic Details
Published inGenomics pp. 173 - 189
Main Authors Charalabous, Panagoula, Woodsmith, Jonathan, Sanderson, Christopher M
Format Book Chapter
LanguageEnglish
Published Chichester, UK John Wiley & Sons, Ltd 19.11.2010
Subjects
activation domain (AD)
Bait and prey auto‐activation tests
binding domain (BD)
Generating recombination‐compatible inserts for gap repair cloning
polymerase chain reaction (PCR)
Targeted ‘matrix’‐style Y2H screens
Using Yeast Two‐Hybrid Methods to Investigate Large Numbers of Binary Protein Interactions
yeast two‐hybrid (Y2H)
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Summary:This chapter contains sections titled: Introduction Methods and approaches Troubleshooting References
ISBN:9780470711576
0470711574
DOI:10.1002/9780470711675.ch8