Effects of IGF-1 and oxLDL on expression of phosphatase PHLPP1 in vascular smooth muscular cells

• Published in: 老年心脏病学杂志（英文版） Vol. 6; no. 4; pp. 237 - 240
• Main Authors: Xing-Li Wu, Ding-You Yang, Zhong-Su Yang, De-Yin Li, Hui-Bin Xu, Shi-Wen Wang
• Format: Journal Article
• Language: English
• Published: Institute of Geriatric Cardiology,Chinese PLA General Hospital,Beijing 100853,China 01.12.2009
• Subjects: insulin-like growth factor-1; PH domain leucine-rich repeat protein phosphatase 1 (PHLPP 1); oxidized low density lipoprotein(oxLDL); vascular smooth muscle cells
• ISSN: 1671-5411

R5; Objective To investigate the effects of insulin-like growth factor-1 (IGF-1) and oxidized low density lipoprotein (oxLDL) on expression of phosphatase PHLPP1 in vascular smooth muscle cells (VSMCs).Methods Rabbit aortic VSMCs were cultured.VSMCs proliferation ability was determined by measuring cell number and mitochondrial dehydrogenase (MD) activity with MTT assay.Western blot was used to detect the protein expression of phosphatase PHLPP 1.Results IGF-1 (100μg/L) increased cell number and MD activity to 3.02 and 3.59 times of that in control group.oxLDL(50μg/ ml) elevated the above two parameters to 2.03 and 2.91 times respectively.Western blot showed that IGF-1 and oxLDL inhibited the expression of PHLPP 1 to 39.27% and 40.26% of the control group (P＜0.01).Conclusion IGF-1 and oxLDL may enhance the proliferation of VSMCs by decreasing the expression of phosphatase PHLPP1 .