沉默CD147基因对姜黄素抑制前列腺癌细胞增殖、迁移、侵袭和诱导凋亡的影响
R735.25; 目的:探讨姜黄素对人前列腺癌C4-2细胞和LNCaP细胞增殖、迁移及侵袭的影响,并阐明其可能的作用机制.方法:采用慢病毒转染系统分别转染C4-2 细胞和LNCaP细胞,作为shCD147-C4-2组和shCD147-LNCaP组.采用RNA干扰技术制备沉默CD147基因细胞,以转入空载体的细胞作为阴性对照,分为shNC-C4-2组(shNC-C4-2细胞)和shNC-LNCaP组(shNC-LNCaP细胞).取生长对数期C4-2、LNCap、shCD147-C4-2和shCD147-LNCaP细胞,加入 20 μmol·L-1 姜黄素,处理 0和 24 h时,显微镜观察各组细...
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| Published in | 吉林大学学报（医学版） Vol. 50; no. 6; pp. 1572 - 1586 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
北华大学基础医学院病原生物学教研室,吉林 吉林 132000%澳门科技大学中医药学院附属医院骨外科,广东 珠海 519003%延边大学医学院生物化学教研室,吉林 延吉 133000
28.11.2024
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| Abstract | R735.25; 目的:探讨姜黄素对人前列腺癌C4-2细胞和LNCaP细胞增殖、迁移及侵袭的影响,并阐明其可能的作用机制.方法:采用慢病毒转染系统分别转染C4-2 细胞和LNCaP细胞,作为shCD147-C4-2组和shCD147-LNCaP组.采用RNA干扰技术制备沉默CD147基因细胞,以转入空载体的细胞作为阴性对照,分为shNC-C4-2组(shNC-C4-2细胞)和shNC-LNCaP组(shNC-LNCaP细胞).取生长对数期C4-2、LNCap、shCD147-C4-2和shCD147-LNCaP细胞,加入 20 μmol·L-1 姜黄素,处理 0和 24 h时,显微镜观察各组细胞形态表现.噻唑蓝(MTT)法检测各组细胞增殖活性,细胞划痕实验检测各组细胞迁移率,Western blotting法检测各组细胞中凋亡、侵袭和迁移相关蛋白表达水平.结果:与C4-2组比较,沉默CD147基因后shCD147-C4-2组细胞中CD147蛋白表达量明显减少;与LNCaP组比较,沉默CD147基因后shCD147-LNCaP组细胞中CD147蛋白表达量明显减少.与处理0 h比较,20 μmol·L-1姜黄素处理24 h后C4-2组和LNCaP组部分细胞出现凋亡征象,且有典型凋亡小体存在;shCD147-C4-2组和shCD147-LNCaP组细胞凋亡现象减弱.MTT法检测,与C4-2+0 μmol·L-1 姜黄素组比较,C4-2+20 μmol·L-1 姜黄素组、C4-2+40 μmol·L-1 姜黄素组、C4-2+60 μmol·L-1 姜黄素组和 C4-2+80 μmol·L-1 姜黄素组细胞增殖活性均明显降低(P<0.01);与LNCaP+0 μmol·L-1姜黄素组比较,LNCaP+20 μmol·L-1姜黄素组、LNCaP+40 μmol·L-1姜黄素组、LNCaP+60 μmol·L-1 姜黄素组和LNCaP+80 μmol·L-1 姜黄素组细胞增殖活性均明显降低(P<0.01);与shNC-C4-2组比较,shNC-C4-2+20 μmol·L-1姜黄素组细胞增殖活性明显降低(P<0.01);与shNC-C4-2+20 μmol·L-1姜黄素组比较,shCD147-C4-2+20 μmol·L-1 姜黄素组细胞增殖活性明显升高(P<0.01);与shNC-LNCaP组比较,shNC-LNCaP+20 |
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| AbstractList | R735.25; 目的:探讨姜黄素对人前列腺癌C4-2细胞和LNCaP细胞增殖、迁移及侵袭的影响,并阐明其可能的作用机制.方法:采用慢病毒转染系统分别转染C4-2 细胞和LNCaP细胞,作为shCD147-C4-2组和shCD147-LNCaP组.采用RNA干扰技术制备沉默CD147基因细胞,以转入空载体的细胞作为阴性对照,分为shNC-C4-2组(shNC-C4-2细胞)和shNC-LNCaP组(shNC-LNCaP细胞).取生长对数期C4-2、LNCap、shCD147-C4-2和shCD147-LNCaP细胞,加入 20 μmol·L-1 姜黄素,处理 0和 24 h时,显微镜观察各组细胞形态表现.噻唑蓝(MTT)法检测各组细胞增殖活性,细胞划痕实验检测各组细胞迁移率,Western blotting法检测各组细胞中凋亡、侵袭和迁移相关蛋白表达水平.结果:与C4-2组比较,沉默CD147基因后shCD147-C4-2组细胞中CD147蛋白表达量明显减少;与LNCaP组比较,沉默CD147基因后shCD147-LNCaP组细胞中CD147蛋白表达量明显减少.与处理0 h比较,20 μmol·L-1姜黄素处理24 h后C4-2组和LNCaP组部分细胞出现凋亡征象,且有典型凋亡小体存在;shCD147-C4-2组和shCD147-LNCaP组细胞凋亡现象减弱.MTT法检测,与C4-2+0 μmol·L-1 姜黄素组比较,C4-2+20 μmol·L-1 姜黄素组、C4-2+40 μmol·L-1 姜黄素组、C4-2+60 μmol·L-1 姜黄素组和 C4-2+80 μmol·L-1 姜黄素组细胞增殖活性均明显降低(P<0.01);与LNCaP+0 μmol·L-1姜黄素组比较,LNCaP+20 μmol·L-1姜黄素组、LNCaP+40 μmol·L-1姜黄素组、LNCaP+60 μmol·L-1 姜黄素组和LNCaP+80 μmol·L-1 姜黄素组细胞增殖活性均明显降低(P<0.01);与shNC-C4-2组比较,shNC-C4-2+20 μmol·L-1姜黄素组细胞增殖活性明显降低(P<0.01);与shNC-C4-2+20 μmol·L-1姜黄素组比较,shCD147-C4-2+20 μmol·L-1 姜黄素组细胞增殖活性明显升高(P<0.01);与shNC-LNCaP组比较,shNC-LNCaP+20 |
| Abstract_FL | Objective:To discuss the effect of curcumin on the proliferation,migration,and invasion of the human prostate cancer C4-2 and LNCaP cells,and to clarify its possible mechanism.Methods:The lentiviral transfection system was used to transfect the C4-2 and LNCaP cells,regarded as shCD147-C4-2 group and shCD147-LNCaP group.RNA interference technology was used to prepare the CD147-silenced cells;the cells transfected with an empty vector were regarded as negative control and divided into shNC-C4-2 group(shNC-C4-2 cells)and shNC-LNCaP group(shNC-LNCaP cells).The C4-2 and LNCaP cells at logarithmic growth phase,as well as shCD147-C4-2 and shCD147-LNCaP cells,were treated with 20 μmol·L-1 curcumin.The morphology of the cells in various groups was observed under microscope at 0 and 24 h of treatment;MTT method was used to detect the proliferation activities of the cells in various groups;cell scratch assay was used to detect the migration rates of the cells in various groups;Western blotting method was used to detect the expression levels of apoptosis,invasion,and migration-related proteins in the cells in various groups.Results:Compared with C4-2 group,the expression of CD147 protein in the cells in shCD147-C4-2 group was significantly decreased after CD147 gene silenting.Compared with LNCaP group,the expression level of CD147 protein in the cells in shCD147-LNCaP group was significantly decreased after CD147 gene silenting.Compared with 0 h of treatment,some cells in C4-2 and LNCaP groups after 24 h of treatment with 20 μmol·L-1 curcumin,showed apoptosis signs with the presence of typical apoptotic bodies.The apoptotic phenomena in shCD147-C4-2 and shCD147-LNCaP groups was reduced.The MTT assay results showed that compared with C4-2+0 μmol·L-1 curcumin group,the proliferation activities of the cells in C4-2+20 μmol·L-1 curcumin group,C4-2+40 μmol·L-1 curcumin group,C4-2+60 μmol·L-1 curcumin group,and C4-2+80 μmol·L-1 curcumin group were decreased(P<0.01).Compared with LNCaP+0 μmol·L-1 curcumin group,the proliferation activity of the cells in LNCaP+20 μ mol·L-1 curcumin group,LNCaP+40 μmol·L-1 curcumin group,LNCaP+60 μmol·L-1 curcumin group,and LNCaP+80 μmol·L-1 curcumin group were decreased(P<0.01).Compared with shNC-C4-2 group,the proliferation activity of the cells in shNC-C4-2+20 μmol·L-1 curcumin group was decreased(P<0.01).Compared with shNC-C4-2+20 μmol·L-1 curcumin group,the proliferation activity of the cells in shCD147-C4-2+20 μmol·L-1 curcumin group was increased(P<0.01).Compared with shNC-LNCaP group,the proliferation activity of the cells in shNC-LNCaP+20 μmol·L-1 curcumin group was decreased(P<0.01);compared with shNC-LNCaP+20 μmol·L-1 curcumin group,the proliferation activity of the cells in shCD147-LNCaP+20 μmol·L-1 curcumin group was significantly increased(P<0.01).The cell scratch healing assay results showed that compared with C4-2 group,the migration rates of the cells in C4-2+20 μmol·L-1 curcumin group and C4-2+40 μmol·L-1 curcumin group after 24 h of treatment were decreased(P<0.01);compared with LNCaP group,the migration rates of the cells in LNCaP+20 μmol·L-1 curcumin group and LNCaP+40 μmol·L-1 curcumin group were increased(P<0.01);compared with shNC-C4-2 group,the migration rate of the cells in shNC-C4-2+20 μmol·L-1 curcumin group was decreased(P<0.01);compared with shNC-C4-2+20 μmol·L-1 curcumin group,the migration rate of the cells in shCD147-C4-2+20 μmol·L-1 curcumin group was significantly increased(P<0.05);compared with shNC-LNCaP group,the migration rate of the cells in shNC-LNCaP+20 μmol·L-1 curcumin group was decreased(P<0.01);compared with shNC-LNCaP+20 μmol·L-1 curcumin group,the garation rate of the cells in shCD147-LNCaP+20 μmol·L-1 curcumin group was significantly increased(P<0.05).The Western blotting results showed that compared with C4-2 group,the expression levels of Bcl-2-associated X protein(Bax),cleaved Caspase-3,and poly ADP-ribose polymerase 1(PARP1)proteins in the cells in C4-2+20 μmol·L-1 curcumin group and C4-2+40 μmol·L-1 curcumin group were significantly increased(P<0.01),and the expression levels of Bcl-2 protein was significantly decreased(P<0.05 or P<0.01);compared with LNCaP group,the expression levels of Bax,cleaved Caspase-3,and PARP1 proteins in the cells in LNCaP+20 μmol·L-1 curcumin group and LNCaP+40 μmol·L-1 curcumin group were significantly increased(P<0.01),and the expression level of Bcl-2 protein in the cells in LNCaP+40 μmol·L-1 curcumin group was decreased(P<0.01);compared with shNC-C4-2 group,the expression levels of Bax,cleaved Caspase-3,and PARP1 proteins in the cells in shNC-C4-2+20 μmol·L-1 curcumin group were significantly increased(P<0.05 or P<0.01),and the expression level of Bcl-2 protein was significantly decreased(P<0.05);compared with shNC-C4-2+20 μmol·L-1 curcumin group,the expression levels of Bax and cleaved Caspase-3 proteins in the cells in shCD147-C4-2+20 μmol·L-1 curcumin group were significantly decreased(P<0.01);compared with shNC-LNCaP group,the expression levels of Bax,cleaved Caspase-3,and PARP1 proteins in the cells in shNC-LNCaP+20 μmol·L-1 curcumin group were significantly increased(P<0.05 or P<0.01),and the expression level of Bcl-2 protein was significantly decreased(P<0.05);compared with shNC-LNCaP+20 μmol·L-1 curcumin group,the expression levels of Bax,cleaved Caspase-3,and PARP1 proteins in the cells in shCD147-LNCaP+20 μmol·L-1 curcumin group were significantly decreased(P<0.05 or P<0.01),and the expression level of Bcl-2 protein was significantly increased(P<0.05).Compared with C4-2 group,the expression levels of E-cadherin protein in the cells in C4-2+20 μmol·L-1 curcumin group and C4-2+40 μ mol·L-1 curcumin group were significantly increased(P<0.01),and the expression levels of N-cadherin and Vimentin proteins were significantly decreased(P<0.01);compared with LNCaP group,the expression levels of E-cadherin protein in the cells in LNCaP+20 μmol·L-1 curcumin group and LNCaP+40 μmol·L-1 curcumin group were significantly increased(P<0.01),and the expression levels of N-cadherin and Vimentin proteins in the cells in LNCaP+40 μmol·L-1 curcumin group were significantly decreased(P<0.01);compared with shNC-C4-2 group,the expression levels of N-cadherin and Vimentin proteins in the cells in shNC-C4-2+20 μmol·L-1 curcumin group were significantly decreased(P<0.01);compared with shNC-C4-2+20 μmol·L-1 curcumin group,the expression level of E-cadherin protein in the cells in shCD147-C4-2+20 μmol·L-1 curcumin group was significantly decreased(P<0.01),and the expression levels of N-cadherin and Vimentin proteins were significantly increased(P<0.01);compared with shNC-LNCaP group,the expression level of E-cadherin protein in the cells in shNC-LNCaP+20 μmol·L-1 curcumin group was significantly increased(P<0.01),and the expression levels of N-cadherin and Vimentin proteins were significantly decreased(P<0.01);compared with shNC-LNCaP+20 μmol·L-1 curcumin group,the expression level of E-cadherin protein in the cells in shCD147-LNCaP+20 μmol·L-1 curcumin group was significantly decreased(P<0.01),and the expression level of N-cadherin was significantly increased(P<0.05).Conclusion:Curcumin inhibits the proliferation,migration,and invasion of the prostate cancer cells in vitro and induces the apoptosis;silencing the CD147 gene partially reduces its inhibitory effect and its ability to induce the apoptosis. |
| Author | 陈姝彤 王馨 侯宗昊 赵杰瑞 郭玉苗 张若文 |
| AuthorAffiliation | 北华大学基础医学院病原生物学教研室,吉林 吉林 132000%澳门科技大学中医药学院附属医院骨外科,广东 珠海 519003%延边大学医学院生物化学教研室,吉林 延吉 133000 |
| AuthorAffiliation_xml | – name: 北华大学基础医学院病原生物学教研室,吉林 吉林 132000%澳门科技大学中医药学院附属医院骨外科,广东 珠海 519003%延边大学医学院生物化学教研室,吉林 延吉 133000 |
| Author_FL | GUO Yumiao ZHANG Ruowen ZHAO Jierui HOU Zonghao WANG Xin CHEN Shutong |
| Author_FL_xml | – sequence: 1 fullname: WANG Xin – sequence: 2 fullname: ZHAO Jierui – sequence: 3 fullname: GUO Yumiao – sequence: 4 fullname: CHEN Shutong – sequence: 5 fullname: HOU Zonghao – sequence: 6 fullname: ZHANG Ruowen |
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| ClassificationCodes | R735.25 |
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| Copyright | Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| DocumentTitle_FL | Effect of silencing CD147 gene on proliferation,migration,invasion,and inducing apoptosis of prostate cancer cells inhibited by curcumin |
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| Keywords | Prostate neoplasm 细胞迁移 Cell invasion Curcumin CD147 姜黄素 前列腺肿瘤 细胞侵袭 Cell migration |
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| PublicationTitle | 吉林大学学报（医学版） |
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| Publisher | 北华大学基础医学院病原生物学教研室,吉林 吉林 132000%澳门科技大学中医药学院附属医院骨外科,广东 珠海 519003%延边大学医学院生物化学教研室,吉林 延吉 133000 |
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| Snippet | R735.25; 目的:探讨姜黄素对人前列腺癌C4-2细胞和LNCaP细胞增殖、迁移及侵袭的影响,并阐明其可能的作用机制.方法:采用慢病毒转染系统分别转染C4-2... |
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| Title | 沉默CD147基因对姜黄素抑制前列腺癌细胞增殖、迁移、侵袭和诱导凋亡的影响 |
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