基于网络药理学和设计空间优化黄芩提取工艺

R284.2; 通过超高效液相色谱-四级杆飞行时间质谱分析黄芩提取液的物质基础,从中鉴定出了 26个黄酮与黄酮醇类化合物,并以药物和疾病为导向,利用网络药理学筛选黄芩苷、野黄芩苷、汉黄芩苷和汉黄芩素为主要活性成分.将这些物质的得率作为提取工艺优化的评价指标,以先水提后醇提的方法,通过确定性筛选实验设计考察工艺参数对评价指标的影响,从而建立黄芩提取的设计空间.实验设计的操作范围为:第1次提取时间为35～45 min,液固比为16～18 mg/L;第2次提取乙醇体积分数为35％～45％,温度为78～82 ℃,时间为45 min,液固比为14～16 mg/L.实验结果表明本文提取工艺稳定可靠,提高了...

Full description

Saved in:
Bibliographic Details
Published in华东理工大学学报（自然科学版） Vol. 50; no. 2; pp. 221 - 230
Main Authors 罗知微, 顾丽芸, 韩伟, 薛东升
Format Journal Article
LanguageChinese
Published 华东理工大学药学院,制药工程与过程化学教育部工程研究中心,上海市新药设计重点实验室,上海 200237%上海凯宝药业股份有限公司,上海 201401 01.05.2024
Subjects
网络药理学
UPLC-MS
network pharmacology
黄芩
超高效液相色谱-质谱联用
提取工艺优化
Scutellariae radix
设计空间
design space
extraction process optimizaiton
Online AccessGet full text

Cover

Abstract R284.2; 通过超高效液相色谱-四级杆飞行时间质谱分析黄芩提取液的物质基础,从中鉴定出了 26个黄酮与黄酮醇类化合物,并以药物和疾病为导向,利用网络药理学筛选黄芩苷、野黄芩苷、汉黄芩苷和汉黄芩素为主要活性成分.将这些物质的得率作为提取工艺优化的评价指标,以先水提后醇提的方法,通过确定性筛选实验设计考察工艺参数对评价指标的影响,从而建立黄芩提取的设计空间.实验设计的操作范围为:第1次提取时间为35～45 min,液固比为16～18 mg/L;第2次提取乙醇体积分数为35％～45％,温度为78～82 ℃,时间为45 min,液固比为14～16 mg/L.实验结果表明本文提取工艺稳定可靠,提高了工艺研究与药物临床的相关性,可为药物质量提升与工艺开发提供思路.
AbstractList R284.2; 通过超高效液相色谱-四级杆飞行时间质谱分析黄芩提取液的物质基础,从中鉴定出了 26个黄酮与黄酮醇类化合物,并以药物和疾病为导向,利用网络药理学筛选黄芩苷、野黄芩苷、汉黄芩苷和汉黄芩素为主要活性成分.将这些物质的得率作为提取工艺优化的评价指标,以先水提后醇提的方法,通过确定性筛选实验设计考察工艺参数对评价指标的影响,从而建立黄芩提取的设计空间.实验设计的操作范围为:第1次提取时间为35～45 min,液固比为16～18 mg/L;第2次提取乙醇体积分数为35％～45％,温度为78～82 ℃,时间为45 min,液固比为14～16 mg/L.实验结果表明本文提取工艺稳定可靠,提高了工艺研究与药物临床的相关性,可为药物质量提升与工艺开发提供思路.
Abstract_FL In this study,ultra performance liquid chromatography-qiadrupole time-of-flight mass spectrometry was used to analyze the chemical components of the extract of Scutellariae radix,from which 26 flavonoids and flacomols were identified.The main active components including baicalin,scutellarin,wogonoside and wogonin were selected by network pharmacology oriented by drug and disease.The yields of these components were used as the evaluation indexes for extraction process optimization.The influence of process parameters on the indexes was investigated through definitive screening design,so as to establish the design space for the extraction of Scutellariae radix.The recommended operating space was as follows:the time and liquid-solid ratio of the first extraction was 35-45 min and 16-18 mg/L,respectively;for the second extraction,the ethanol volume fraction was 35％—45％,the temperature was 78-82 ℃,the extraction time was 45 min and the liquid-solid ratio was 14-16 mg/L.The extraction process was proved to be stable and reliable.This study has improved the correlation between process research and drug clinical practice,which provided ideas for actual quality improvement and process development.
Author 薛东升
韩伟
罗知微
顾丽芸
AuthorAffiliation 华东理工大学药学院,制药工程与过程化学教育部工程研究中心,上海市新药设计重点实验室,上海 200237%上海凯宝药业股份有限公司,上海 201401
AuthorAffiliation_xml – name: 华东理工大学药学院,制药工程与过程化学教育部工程研究中心,上海市新药设计重点实验室,上海 200237%上海凯宝药业股份有限公司,上海 201401
Author_FL LUO Zhiwei
GU Liyun
XUE Dongsheng
HAN Wei
Author_FL_xml – sequence: 1
  fullname: LUO Zhiwei
– sequence: 2
  fullname: GU Liyun
– sequence: 3
  fullname: HAN Wei
– sequence: 4
  fullname: XUE Dongsheng
Author_xml – sequence: 1
  fullname: 罗知微
– sequence: 2
  fullname: 顾丽芸
– sequence: 3
  fullname: 韩伟
– sequence: 4
  fullname: 薛东升
BookMark eNo9j7tKA0EYhaeIYIx5DAuLXf-57OwM2EjwEgjYaB1md2djYpiAi5gHEEGJJlpEC0EsFFIYG0HYqE-TcTdvYUCxOnA-OB9nCRVMx2iEVjC4mGHqrbXc0Bw2XQzAHQoCXAKEYEIYAC6g4n-_iMpJ0gxgTj2BOS6idfuQTtOr7PM6m9znl6_Z4My-PNubXj7-yseP2Sid3b5NP-5sbzibnOYXo-_-wPaH9v0pP0-X0UKs2oku_2UJ7W9t7lV2nNrudrWyUXMSDEw6inoBKE4Fj0nIPQk8ZJEMNNGRL3wgvmJKax6CZirW2FfSD6mIQiKViKWktIRWf3dPlImVadRbneMjMzfWD6J2I-p2g_lfBgRA0h8XwmUM
ClassificationCodes R284.2
ContentType Journal Article
Copyright Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
Copyright_xml – notice: Copyright © Wanfang Data Co. Ltd. All Rights Reserved.
DBID 2B.
4A8
92I
93N
PSX
TCJ
DOI 10.14135/j.cnki.1006-3080.20221224001
DatabaseName Wanfang Data Journals - Hong Kong
WANFANG Data Centre
Wanfang Data Journals
万方数据期刊 - 香港版
China Online Journals (COJ)
China Online Journals (COJ)
DatabaseTitleList
DeliveryMethod fulltext_linktorsrc
Discipline Engineering
DocumentTitle_FL Optimization of Extraction Process of Scutellariae radix Based on Network Pharmacology and Design Space
EndPage 230
ExternalDocumentID hdlgdxxb202402009
GrantInformation_xml – fundername: 国家中医药管理局项目
  funderid: (ZYBZH-C-SH-48)
GroupedDBID -02
2B.
4A8
92I
93N
ALMA_UNASSIGNED_HOLDINGS
CCEZO
CDRFL
P2P
PSX
TCJ
U1G
U5L
UY8
ID FETCH-LOGICAL-s1049-a35b0a6386f2c65906c4d9be2ed787027a4aee6c0e4afe17a97c38dc29a8f9933
ISSN 1006-3080
IngestDate Thu May 29 03:59:03 EDT 2025
IsPeerReviewed true
IsScholarly true
Issue 2
Keywords 网络药理学
UPLC-MS
network pharmacology
黄芩
超高效液相色谱-质谱联用
提取工艺优化
Scutellariae radix
设计空间
design space
extraction process optimizaiton
Language Chinese
LinkModel OpenURL
MergedId FETCHMERGED-LOGICAL-s1049-a35b0a6386f2c65906c4d9be2ed787027a4aee6c0e4afe17a97c38dc29a8f9933
PageCount 10
ParticipantIDs wanfang_journals_hdlgdxxb202402009
PublicationCentury 2000
PublicationDate 2024-05-01
PublicationDateYYYYMMDD 2024-05-01
PublicationDate_xml – month: 05
  year: 2024
  text: 2024-05-01
  day: 01
PublicationDecade 2020
PublicationTitle 华东理工大学学报（自然科学版）
PublicationTitle_FL Journal of East China University of Science and Technology(Natural Science Edition)
PublicationYear 2024
Publisher 华东理工大学药学院,制药工程与过程化学教育部工程研究中心,上海市新药设计重点实验室,上海 200237%上海凯宝药业股份有限公司,上海 201401
Publisher_xml – name: 华东理工大学药学院,制药工程与过程化学教育部工程研究中心,上海市新药设计重点实验室,上海 200237%上海凯宝药业股份有限公司,上海 201401
SSID ssib002258161
ssib001104923
ssib057620135
ssib035757162
ssib036434644
ssib006703735
ssib000947709
ssib006595864
ssib051370438
ssib023167207
ssib001050798
ssj0039901
Score 2.3752415
Snippet R284.2; 通过超高效液相色谱-四级杆飞行时间质谱分析黄芩提取液的物质基础,从中鉴定出了...
SourceID wanfang
SourceType Aggregation Database
StartPage 221
Title 基于网络药理学和设计空间优化黄芩提取工艺
URI https://d.wanfangdata.com.cn/periodical/hdlgdxxb202402009
Volume 50
hasFullText 1
inHoldings 1
isFullTextHit
isPrint
link http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3Na9RAFA-lBdGD-InfFHGOW5PZSTIDXpJuQhHrxRZ6K0k224qygm2h9CyCUm31UD0I4kGhB-tFEFr1r-m62__C914myZRWqV7C7OR9_PLem933ZmcmlnXDybmQ3Ong-XZZQ4gUxpzdsRtpmmWO6Lhe28bdyJN3vYlpcXvGnRkamTNWLS0tpmPZyqH7Sv7Hq9AHfsVdsv_g2UoodEAb_AtX8DBcj-RjFrlMxSwMWCTwKiMW-SxsMeVQI2RqnEWSyRYLYuxRNpMecgXQQw3FmSSaIGJhpBsBsQeKJCumQJQgFeNMSeQCFuXhLVAhBakIkD7ymIxRC9LEROOyEES5RIMCzWyYyFoEG4RLQmuA1IyAVrDA3wdbNzzSCzQxYpOStABXgHIAWOjRg_hkkIrLRyRIXHBVUyGl8XxCEWvlYJUgqkkUWicsEQN58fChNEmQWZUWi-s7Ek2iQuNxCwP45uQLF_VSRxou_2umyvFVDyAD_BzGFcmUZCCDTIuCK3SWMCWFRRijZetbVRAYzlAuykeBnIUc1ckm0h8iGRo2U6KMM0_rAlFIHCMjgiwABCg8JCF4l-SjOui0DfwHg1ghZriLLgdIitBGTEXkx4BCn3rAdIepwwM3OR4X4R4OBWMtLoW2DCiFnwJtC4QicKxguHjoPwjAwhl6rICEcT1ogPEPUHCWwkgibFpPWryhrMwyiuOV9bcpN1OG4oQAnX3y4l_KA4kN5JouZTZZ98H9sUrDGAQld2ghuFNndNU62_n2w7n28nKKoWsXG51HONTTfNgaCVqTd-4ZlZbw953kaEOhaMzEQJUgzJkWSAOkY2yJx2NJzRc9eJBJ-HUlxvHADV7PNDWhSizPrqPPUBgJr640XKfp4xqF6jPkbFCsVjMbTfw7nxayaEMcs1hpppt_MxLtzex2ku6cUUZMnbJO6vp_NCi-zE9bQyvzZ6wTxqmwZ61bvffbu9sv-z9e9XfeDV586a8_7X3-1Hu9Otj6Odj60N_c3nvzdff7297qxt7Ok8HzzV9r6721jd63j4Nn2-es6TiaGp9o6JfcNBbQpI2k6aZ2AlmQ1-EZWNH2MtFWac7zNuZS3E9EkudeZuci6eSOnyg_a8p2xlUiO1BcNs9bw91H3fyCNeq1k1ylCWRtIhfSd5KOTS-VzGwHfnpVftG6rp99Vv-ILcweiJBLRyG6bB2vvw2vWMOLj5fyq1CcLabXdGD9BmUVFSE
linkProvider EBSCOhost
openUrl ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=%E5%9F%BA%E4%BA%8E%E7%BD%91%E7%BB%9C%E8%8D%AF%E7%90%86%E5%AD%A6%E5%92%8C%E8%AE%BE%E8%AE%A1%E7%A9%BA%E9%97%B4%E4%BC%98%E5%8C%96%E9%BB%84%E8%8A%A9%E6%8F%90%E5%8F%96%E5%B7%A5%E8%89%BA&rft.jtitle=%E5%8D%8E%E4%B8%9C%E7%90%86%E5%B7%A5%E5%A4%A7%E5%AD%A6%E5%AD%A6%E6%8A%A5%EF%BC%88%E8%87%AA%E7%84%B6%E7%A7%91%E5%AD%A6%E7%89%88%EF%BC%89&rft.au=%E7%BD%97%E7%9F%A5%E5%BE%AE&rft.au=%E9%A1%BE%E4%B8%BD%E8%8A%B8&rft.au=%E9%9F%A9%E4%BC%9F&rft.au=%E8%96%9B%E4%B8%9C%E5%8D%87&rft.date=2024-05-01&rft.pub=%E5%8D%8E%E4%B8%9C%E7%90%86%E5%B7%A5%E5%A4%A7%E5%AD%A6%E8%8D%AF%E5%AD%A6%E9%99%A2%2C%E5%88%B6%E8%8D%AF%E5%B7%A5%E7%A8%8B%E4%B8%8E%E8%BF%87%E7%A8%8B%E5%8C%96%E5%AD%A6%E6%95%99%E8%82%B2%E9%83%A8%E5%B7%A5%E7%A8%8B%E7%A0%94%E7%A9%B6%E4%B8%AD%E5%BF%83%2C%E4%B8%8A%E6%B5%B7%E5%B8%82%E6%96%B0%E8%8D%AF%E8%AE%BE%E8%AE%A1%E9%87%8D%E7%82%B9%E5%AE%9E%E9%AA%8C%E5%AE%A4%2C%E4%B8%8A%E6%B5%B7+200237%25%E4%B8%8A%E6%B5%B7%E5%87%AF%E5%AE%9D%E8%8D%AF%E4%B8%9A%E8%82%A1%E4%BB%BD%E6%9C%89%E9%99%90%E5%85%AC%E5%8F%B8%2C%E4%B8%8A%E6%B5%B7+201401&rft.issn=1006-3080&rft.volume=50&rft.issue=2&rft.spage=221&rft.epage=230&rft_id=info:doi/10.14135%2Fj.cnki.1006-3080.20221224001&rft.externalDocID=hdlgdxxb202402009
thumbnail_s http://utb.summon.serialssolutions.com/2.0.0/image/custom?url=http%3A%2F%2Fwww.wanfangdata.com.cn%2Fimages%2FPeriodicalImages%2Fhdlgdxxb%2Fhdlgdxxb.jpg