普通丝瓜PYL基因家族鉴定及其在果实发育中的表达分析
Q786%S642.4; [目的]探究普通丝瓜PYL基因在ABA处理下的表达模式,以期为深入研究其基因功能提供参考依据,也为分子抗性育种以及提高普通丝瓜品质和产量提供新思路.[方法]用生物信息学方法,对普通丝瓜PYL基因家族进行全基因组鉴定,染色体定位,以及基因结构、基因共线性、选择压力、进化亲缘关系和顺式作用元件分析,并通过qRT-PCR验证在不同浓度ABA下基因特征表达.[结果]在普通丝瓜全基因组中鉴定到11个LcPYL基因,CDS长度576～966 bp,编码蛋白均具有典型PYR_PYL_RCAR_like结构;共线性分析发现7对片段复制基因对,普通丝瓜PYL基因启动子含多个与植物激素相...
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| Published in | 西北植物学报 Vol. 44; no. 3; pp. 408 - 420 |
|---|---|
| Main Authors | , , , , , |
| Format | Journal Article |
| Language | Chinese |
| Published |
福建省农业科学院作物研究所,福州 350013
2024
福建农林大学园艺学院,福州 350002%福建农林大学园艺学院,福州 350002 福建省蔬菜遗传育种重点实验室,福州 350013%福建省农业科学院作物研究所,福州 350013 福建省蔬菜遗传育种重点实验室,福州 350013 |
| Subjects | |
| Online Access | Get full text |
| ISSN | 1000-4025 |
| DOI | 10.7606/j.issn.1000-4025.20230546 |
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| Abstract | Q786%S642.4; [目的]探究普通丝瓜PYL基因在ABA处理下的表达模式,以期为深入研究其基因功能提供参考依据,也为分子抗性育种以及提高普通丝瓜品质和产量提供新思路.[方法]用生物信息学方法,对普通丝瓜PYL基因家族进行全基因组鉴定,染色体定位,以及基因结构、基因共线性、选择压力、进化亲缘关系和顺式作用元件分析,并通过qRT-PCR验证在不同浓度ABA下基因特征表达.[结果]在普通丝瓜全基因组中鉴定到11个LcPYL基因,CDS长度576～966 bp,编码蛋白均具有典型PYR_PYL_RCAR_like结构;共线性分析发现7对片段复制基因对,普通丝瓜PYL基因启动子含多个与植物激素相关的顺式作用元件,其成员的表达具有一定的组织特异性;普通丝瓜果实在不同浓度外源ABA处理下:2 mg/mL ABA处理LcPYLs表达无显著变化,4 mg/mL ABA处理LcPYL5、LcPYL7、LcPYL11显著上调表达,8 mg/mL ABA处理LcPYL1、LcPYL7开花当天显著表达.[结论]对普通丝瓜PYL家族成员进行了系统的鉴定与分析,明确其分子结构特征和表达模式,其中LcPYL1、LcPYL5、LcPYL7和LcPYL1 1可能是与内源ABA生物合成密切相关的关键基因. |
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| AbstractList | Q786%S642.4; [目的]探究普通丝瓜PYL基因在ABA处理下的表达模式,以期为深入研究其基因功能提供参考依据,也为分子抗性育种以及提高普通丝瓜品质和产量提供新思路.[方法]用生物信息学方法,对普通丝瓜PYL基因家族进行全基因组鉴定,染色体定位,以及基因结构、基因共线性、选择压力、进化亲缘关系和顺式作用元件分析,并通过qRT-PCR验证在不同浓度ABA下基因特征表达.[结果]在普通丝瓜全基因组中鉴定到11个LcPYL基因,CDS长度576～966 bp,编码蛋白均具有典型PYR_PYL_RCAR_like结构;共线性分析发现7对片段复制基因对,普通丝瓜PYL基因启动子含多个与植物激素相关的顺式作用元件,其成员的表达具有一定的组织特异性;普通丝瓜果实在不同浓度外源ABA处理下:2 mg/mL ABA处理LcPYLs表达无显著变化,4 mg/mL ABA处理LcPYL5、LcPYL7、LcPYL11显著上调表达,8 mg/mL ABA处理LcPYL1、LcPYL7开花当天显著表达.[结论]对普通丝瓜PYL家族成员进行了系统的鉴定与分析,明确其分子结构特征和表达模式,其中LcPYL1、LcPYL5、LcPYL7和LcPYL1 1可能是与内源ABA生物合成密切相关的关键基因. |
| Abstract_FL | [Objective]Expression of PYL genes in Luffa cylindrica under ABA treatment was studied to provide a reference for gene function and to provide foundation for molecular breeding to improve the quali-ty and yield of L.cylindrica.[Methods]Bioinformatics methods were used to analyze the distribution,chromosome location,gene structure,gene collinearity,selection pressure,evolutionary relationships,and cis-acting elements of the PYL gene family in L.cylindrica.Expression of the PYL gene family un-der different concentrations of ABA was verified by qRT-PCR.[Results]Eleven LcPYL genes were identi-fied in the genome of L.cylindrica.LcPYL genes had CDSs of 576-966 bp with typical PYRPYLRCAR_like structures.Collinear analysis identified seven repeating gene pairs that were predicted to have similar functions.LcPYL promoters contained cis-acting elements associated with plant hormones,and the ex-pression of its members was tissue-specific.Expression of LcPYLs in L.cylindrica fruits did not change at 2 mg/mL ABA.However,the expression of LcPYL5,LcPYL7,and LcPYLl1 was significantly in-creased at 4 mg/mL ABA,while LcPYL1 and LcPYL7 were expressed at the day of flowering at 8 mg/mL ABA.[Conclusion]Our findings suggest that LcPYL1,LcPYL5,LcPYL7,and LcPYL11 may be closely linked to endogenous ABA biosynthesis. |
| Author | 温庆放 朱海生 钟凤林 王玉倩 刘建汀 彭丽娟 |
| AuthorAffiliation | 福建农林大学园艺学院,福州 350002%福建农林大学园艺学院,福州 350002;福建省农业科学院作物研究所,福州 350013;福建省蔬菜遗传育种重点实验室,福州 350013%福建省农业科学院作物研究所,福州 350013;福建省蔬菜遗传育种重点实验室,福州 350013 |
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| Author_FL | ZHU Haisheng WEN Qingfang ZHONG Fenglin WANG Yuqian PENG Lijuan LIU Jianting |
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| DocumentTitle_FL | Genome-wide identification of the PYL gene family in Luff a cylindrica and expression analysis in fruit development |
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| Keywords | Luffa cylindrica PYL gene family fruit development PYL基因家族 ABA treatment expression analysis 外源ABA处理 表达分析 果实发育 普通丝瓜 |
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| Title | 普通丝瓜PYL基因家族鉴定及其在果实发育中的表达分析 |
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